ArticleBiology of reproduction2018
Glutamine supplementation enhances development of in vitro-produced porcine embryos and increases leucine consumption from the medium.
Article in Biology of reproduction, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- Phenotyping of post-fertilization sperm mitophagy determinants discovered in a mammalian gamete-based cell-free system.Biological research · 2026Article
- Dynamics of the Mammalian Placental Metabolome in Placentogenesis and Embryonic Development.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The Critical Functions of FGF2, LIF and IGF1 in the Improvement of In Vitro Embryo Production.Biomolecules · 2026Review
- Excess glutamine in embryo culture media alters mitochondrial and autophagy-related dynamics associated with impaired postnatal development.Frontiers in cell and developmental biology · 2026Article
- Article
- Review
- Transcriptional profiling reveals upregulation of p53 signaling in porcine embryos produced in vitro†.Biology of reproduction · 2025Article
- Expression of tripartite motif family-like protein 1 and 2 in early conceptus development and placentation in the pig.Reproduction & fertility · 2025Article
- Development and Initial Characterization of Pigs withbioRxiv : the preprint server for biology · 2024Article
- Novel off-Targeting Events Identified after Genome Wide Analysis of CRISPR-Cas Edited Pigs.The CRISPR journal · 2024Article
- Revealing the pathogenesis of gastric intestinal metaplasia based on the mucosoid air-liquid interface.Journal of translational medicine · 2024Article
- Metabolomics Integration in Assisted Reproductive Technologies for Enhanced Embryo Selection beyond Morphokinetic Analysis.International journal of molecular sciences · 2023Article
- Metabolomic Profiling of Female Mink Serum during Early to Mid-Pregnancy to Reveal Metabolite Changes.Genes · 2023Article
- Inactivation of growth differentiation factor 9 blocks folliculogenesis in pigs†.Biology of reproduction · 2023Article
- Metabonomic analysis of follicular fluid in patients with diminished ovarian reserve.Frontiers in endocrinology · 2023Article
- Glutamine as a Potential Noninvasive Biomarker for Human Embryo Selection.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Review
- Effects of RAD51-stimulatory compound 1 (RS-1) and its vehicle, DMSO, on pig embryo culture.Reproductive toxicology (Elmsford, N.Y.) · 2021Article
- Glutaminolysis is involved in the activation of mTORC1 in in vitro-produced porcine embryos.Molecular reproduction and development · 2021Article
- Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Improper composition of culture medium contributes to reduced viability of in vitro-produced embryos. Glutamine (Gln) is a crucial amino acid for preimplantation embryos as it supports proliferation and is involved in many different biosynthetic pathways. Previous transcriptional profiling revealed several upregulated genes related to Gln transport and metabolism in in vitro-produced porcine blastocysts compared to in vivo-produced counterparts, indicating a potential deficiency in the culture medium. Therefore, the objective of this study was to determine the effects of Gln supplementation on in vitro-produced porcine embryo development, gene expression, and metabolism. Cleaved embryos were selected and cultured in MU2 medium supplemented with 1 mM Gln (control), 3.75 mM Gln (+Gln), 3.75 mM GlutaMAX (+Max), or 3.75 mM alanine (+Ala) until day 6. Embryos cultured with +Gln or +Max had increased development to the blastocyst stage and total number of nuclei compared to the control (P < 0.05). Moreover, expression of misregulated transcripts involved in glutamine and glutamate transport and metabolism was corrected when embryos were cultured with +Gln or +Max. Metabolomics analysis revealed increased production of glutamine and glutamate into the medium by embryos cultured with +Max and increased consumption of leucine by embryos cultured with +Gln or +Max. As an indicator of cellular health, mitochondrial membrane potential was increased when embryos were cultured with +Max which was coincident with decreased apoptosis in these blastocysts. Lastly, two embryo transfers by using embryos cultured with +Max resulted in viable piglets, confirming that this treatment is consistent with in vivo developmental competence.
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