ArticleReproductive biology and endocrinology : RB&E2018
The thermo-sensitive gene expression signatures of spermatogenesis.
Article in Reproductive biology and endocrinology : RB&E, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07315737 (miRNAs Associated With Undescended and Retractile Testes), which is not on this map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
miRNAs Associated With Undescended and Retractile Testes
Who cites it
34 citing papers in PubMed, 72 citations in OpenAlex.
- Could miRNAs be used as markers for distinguishing undescended testes from retractile testes? A prospective controlled pilot/exploratory study.BMC urology · 2026Observational
- The RNA helicase DDX53 (CAGE) contributes to RNA metabolism in a human germ cell model.bioRxiv : the preprint server for biology · 2026Article
- Global Warming, Fertility, and Spermatogenesis Decline: Global and Regional Evidence from 195 Countries and Implications for Climate Adaptation Policy.International journal of environmental research and public health · 2026Article
- Acute testicular hyperthermia leads to rapid loss of global piRNA and a concurrent increase in LINE1 activity within heat sensitive male germ cells.Mobile DNA · 2026Article
- Comparative analysis of ERK and CREB activity in normal and cryptorchid testes of bulls.Journal of veterinary science · 2025Article
- Astaxanthin Alleviates the Decline of Sperm Quality Caused by Heat Stress in Mice via Reducing Oxidative Stress.Life (Basel, Switzerland) · 2025Article
- Whole-Genome Sequencing Identifies Functional Genes for Environmental Adaptability in Chinese Geese.Animals : an open access journal from MDPI · 2025Article
- The involvement of TRPV1 in the apoptosis of spermatogenic cells in the testis of mice with cryptorchidism.Cell death discovery · 2025Article
- Environmentally induced variation in sperm sRNAs is linked to gene expression and transposable elements in zebrafish offspring.Heredity · 2025Article
- Semen Quality, Testicular Cell Apoptosis, and Transcriptome Analysis Following Mild Scrotal Heat Stress in Wugu-Hu Crossbred and Hu Rams.Animals : an open access journal from MDPI · 2025Article
- Identification of key genes related to unilateral cryptorchidism in sheep by comprehensive transcriptomics and proteomics analyses.BMC genomics · 2025Article
- Review of the Different Outcomes Produced by Genetic Knock Out of the Long Non-coding microRNA-host-gene MIR22HGMicroRNA (Shariqah, United Arab Emirates) · 2025Review
- Neonatal exposure to high D-galactose affects germ cell development in neonatal testes organ culture.Scientific reports · 2024Article
- MicroRNA Analysis of In Vitro Differentiation of Spermatogonial Stem Cells Using a 3D Human Testis Organoid System.Biomedicines · 2024Article
- Heat stress induced piRNA alterations in pachytene spermatocytes and round spermatids.Reproductive biology and endocrinology : RB&E · 2024Article
- Therapeutic Effects of Exosome Therapy and Photobiomodulation Therapy on the Spermatogenesis Arrest in Male Mice After Scrotum Hyperthermia.Journal of lasers in medical sciences · 2024Article
- MAPKs signaling is obligatory for male reproductive function in a development-specific manner.Frontiers in reproductive health · 2024Review
- Heat stress and stallion fertility.Journal of animal science and technology · 2023Review
- Whole genome analyses reveal novel genes associated with chicken adaptation to tropical and frigid environments.Journal of advanced research · 2023Article
- STUB1 directs FOXQ1-mediated transactivation of Ldha gene and facilitates lactate production in mouse Sertoli cells.Cell and tissue research · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSpermatogenesis in most mammals (including human and rat) occurs at ~ 3 °C lower than body temperature in a scrotum and fails rapidly at 37 °C inside the abdomen. The present study investigates the heat-sensitive transcriptome and miRNAs in the most vulnerable germ cells (spermatocytes and round spermatids) that are primarily targeted at elevated temperature in a bid to identify novel targets for contraception and/or infertility treatment.
methodsTestes of adult male rats subjected to surgical cryptorchidism were obtained at 0, 24, 72 and 120 h post-surgery, followed by isolation of primary spermatocytes and round spermatids and purification to > 90% purity using a combination of trypsin digestion, centrifugal elutriation and density gradient centrifugation techniques. RNA isolated from these cells was sequenced by massive parallel sequencing technique to identify the most-heat sensitive mRNAs and miRNAs.
resultsHeat stress altered the expression of a large number of genes by ≥2.0 fold, out of which 594 genes (286↑; 308↓) showed alterations in spermatocytes and 154 genes (105↑; 49↓) showed alterations in spermatids throughout the duration of experiment. 62 heat-sensitive genes were common to both cell types. Similarly, 66 and 60 heat-sensitive miRNAs in spermatocytes and spermatids, respectively, were affected by ≥1.5 fold, out of which 6 were common to both the cell types.
conclusionThe study has identified Acly, selV, SLC16A7(MCT-2), Txnrd1 and Prkar2B as potential heat sensitive targets in germ cells, which may be tightly regulated by heat sensitive miRNAs rno-miR-22-3P, rno-miR-22-5P, rno-miR-129-5P, rno-miR-3560, rno-miR-3560 and rno-miR-466c-5P.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.