ReviewBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2018
Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast.
Review in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- A Systematic Review of the Potential of Pichia pastoris (Komagataella phaffii) as an Alternative Host for Biologics Production.Molecular biotechnology · 2024Pooled it
- Pichia Toolkit: Use of the combinatorial library screening system for expression of a marine laccase.Folia microbiologica · 2026Article
- Metabolic Engineering ofMolecules (Basel, Switzerland) · 2024Article
- Coupling thermotolerance and high production of recombinant protein by CYR1Communications biology · 2024Article
- Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production.Biotechnology for biofuels and bioproducts · 2023Article
- Improving Methanol Utilization by Reducing Alcohol Oxidase Activity and Adding Co-Substrate of Sodium Citrate inJournal of fungi (Basel, Switzerland) · 2023Article
- Applicability of the heterologous yeast promoters for recombinant protein production in Pichia pastoris.Applied microbiology and biotechnology · 2022Article
- Effects of Temperature and pH on Recombinant Thaumatin II Production byFoods (Basel, Switzerland) · 2022Article
- Comparative transcriptome and metabolome analyses reveal the methanol dissimilation pathway of Pichia pastoris.BMC genomics · 2022Article
- Advances in Cell Engineering of theMetabolites · 2022Review
- Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins.Applied microbiology and biotechnology · 2021Review
- Enhanced Recombinant Protein Production Under Special Environmental Stress.Frontiers in microbiology · 2021Review
- Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others; but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this review focuses on the selection of the best promoter in the recombination process, considering different inductors, and the temperature as a culture medium variable in methylotrophic Pichia pastoris yeast. The goal is to understand the effects associated with different factors that influence its cell metabolism and to reach the construction of an expression system that fulfills the requirements of the yeast, presenting an optimal growth and development in batch, fed-batch or continuous cultures, and at the same time improve its yield in heterologous protein production.
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Registered trials
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