Evidence map›Paper›PMID 29857551›Full record

ArticleMolecules (Basel, Switzerland)2018

Replacing Standard Reporters from Molecular Cloning Plasmids with Chromoproteins for Positive Clone Selection.

Margarita Daniela Tafoya-Ramírez, Felipe Padilla-Vaca, Ana Patricia Ramírez-Saldaña, Josué Daniel Mora-Garduño, Ángeles Rangel-Serrano, Naurú Idalia Vargas-Maya, Luz Janeth Herrera-Gutiérrez, Bernardo Franco

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
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  4. Article
  5. A Strategy for the Rapid Development of a SafeInternational journal of molecular sciences · 2021
    Article
  6. aeBlue Chromoprotein Color is Temperature Dependent.Protein and peptide letters · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Margarita Daniela Tafoya-RamírezDepartamento de Biología, Universidad de Guanajuato, Noria Alta, 36050 Guanajuato, Mexico. max_tafas@hotmail.com.
Felipe Padilla-VacaDepartamento de Biología, Universidad de Guanajuato, Noria Alta, 36050 Guanajuato, Mexico. padillaf@ugto.mx.
Ana Patricia Ramírez-SaldañaDepartamento de Biología, Universidad de Guanajuato, Noria Alta, 36050 Guanajuato, Mexico. anapatricia.rs.18@gmail.com.
Josué Daniel Mora-GarduñoDepartamento de Biología, Universidad de Guanajuato, Noria Alta, 36050 Guanajuato, Mexico. jhosdj@live.com.mx.
Ángeles Rangel-SerranoDepartamento de Biología, Universidad de Guanajuato, Noria Alta, 36050 Guanajuato, Mexico. arangel@ugto.mx.
Naurú Idalia Vargas-MayaDepartamento de Biología, Universidad de Guanajuato, Noria Alta, 36050 Guanajuato, Mexico. naudalia@hotmail.com.
Luz Janeth Herrera-GutiérrezDepartamento de Biología, Universidad de Guanajuato, Noria Alta, 36050 Guanajuato, Mexico. luz.janeth.herrera@hotmail.com.
Bernardo FrancoDepartamento de Biología, Universidad de Guanajuato, Noria Alta, 36050 Guanajuato, Mexico. bfranco@ugto.mx.
Universidad de Guanajuato · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cloning and expression plasmids are the workhorses of modern molecular biology. Despite the pathway paved by synthetic biology, laboratories around the globe still relay on standard cloning techniques using plasmids with reporter proteins for positive clone selection, such as β-galactosidase alpha peptide complementation for blue/white screening or

Indexed as

Clonal EvolutionCloning, MolecularGene ExpressionBacterial ProteinsGene OrderGenes, ReporterModels, MolecularPlasmidsProtein ConformationBacterial ProteinsAmilCP chromoproteinclone selectionmolecular cloningplasmidprotein expression

Identifiers

PMID29857551
PMCPMC6099721
OpenAlexW2805651201

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.