ArticleRapid communications in mass spectrometry : RCM2018
Peptidomic analysis of endogenous plasma peptides from patients with pancreatic neuroendocrine tumours.
Article in Rapid communications in mass spectrometry : RCM, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Small-protein Enrichment Assay Enables the Rapid, Unbiased Analysis of Over 100 Low Abundance Factors from Human Plasma.Molecular & cellular proteomics : MCP · 2019Trial
- Multi-omic profiles of neuroendocrine neoplasms of the pancreas: an integrated landscape.Molecular cancer · 2026Review
- Identification of intestinal enteroendocrine cell subtypes and their associated hormones in zebrafish.PLoS biology · 2025Article
- Glucagonoma and Glucagonoma Syndrome: An Updated Review.Clinical endocrinology · 2025Review
- Development and Validation of a Capillary Electrophoresis Coupled to Mass Spectrometry Pipeline for Comparable Assessment of the Plasma Peptidome.Proteomics · 2025Article
- Article
- Clinical Peptidomics in Acute Leukemias: Current Advances and Future Perspectives.Journal of proteome research · 2024Review
- An unwelcome inheritance: childhood obesity after diabetes in pregnancy.Diabetologia · 2023Review
- Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples.Frontiers in endocrinology · 2023Article
- Clinical Peptidomics: Advances in Instrumentation, Analyses, and Applications.BME frontiers · 2023Review
- Obesogenic diet in mice compromises maternal metabolic physiology and lactation ability leading to reductions in neonatal viability.Acta physiologica (Oxford, England) · 2022Article
- Solvent Precipitation SP3 (SP4) Enhances Recovery for Proteomics Sample Preparation without Magnetic Beads.Analytical chemistry · 2022Article
- Acanthosis nigricans in a patient with metastatic insulinoma post peptide receptor radionuclide therapy.Endocrinology, diabetes & metabolism case reports · 2022Article
- Increased C-Peptide Immunoreactivity in Insulin Autoimmune Syndrome (Hirata Disease) Due to High Molecular Weight Proinsulin.Clinical chemistry · 2021Article
- Metabolic Messengers: glucagon-like peptide 1.Nature metabolism · 2021Review
- Mass spectrometric characterisation of the circulating peptidome following oral glucose ingestion in control and gastrectomised patients.Rapid communications in mass spectrometry : RCM · 2020Article
- Article
- Single cell transcriptomic profiling of large intestinal enteroendocrine cells in mice - Identification of selective stimuli for insulin-like peptide-5 and glucagon-like peptide-1 co-expressing cells.Molecular metabolism · 2019Article
- Characterisation of proguanylin expressing cells in the intestine - evidence for constitutive luminal secretion.Scientific reports · 2019Article
- Glucagon Receptor Signaling and Glucagon Resistance.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
rationaleDiagnosis of pancreatic neuroendocrine tumours requires the study of patient plasma with multiple immunoassays, using multiple aliquots of plasma. The application of mass spectrometry based techniques could reduce the cost and amount of plasma required for diagnosis.
methodsPlasma samples from two patients with pancreatic neuroendocrine tumours were extracted using an established acetonitrile-based plasma peptide enrichment strategy. The circulating peptidome was characterised using nano and high flow rate liquid chromatography/mass spectrometry (LC/MS) analyses. To assess the diagnostic potential of the analytical approach, a large sample batch (68 plasmas) from control subjects, and aliquots from subjects harbouring two different types of pancreatic neuroendocrine tumour (insulinoma and glucagonoma), were analysed using a 10-min LC/MS peptide screen.
resultsThe untargeted plasma peptidomics approach identified peptides derived from the glucagon prohormone, chromogranin A, chromogranin B and other peptide hormones and proteins related to control of peptide secretion. The glucagon prohormone derived peptides that were detected were compared against putative peptides that were identified using multiple antibody pairs against glucagon peptides. Comparison of the plasma samples for relative levels of selected peptides showed clear separation between the glucagonoma and the insulinoma and control samples.
conclusionsThe combination of the organic solvent extraction methodology with high flow rate analysis could potentially be used to aid diagnosis and monitor treatment of patients with functioning pancreatic neuroendocrine tumours. However, significant validation will be required before this approach can be clinically applied.
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