Evidence map›Paper›PMID 29857015›Full record

ArticleNeuroscience research2019

In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons.

Tianxiang Huang, Lena H Nguyen, Tiffany V Lin, Xuan Gong, Longbo Zhang, Gi Bum Kim, Matthew R Sarkisian, Joshua J Breunig, Angelique Bordey

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Tianxiang HuangDepartment of Neurosurgery, Xiangya Hospital, Central South University, 85 Xiangya Street, Changsha, 410008, China; Departments of Neurosurgery, and Cellular & Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06520-8082, USA.
Lena H NguyenDepartments of Neurosurgery, and Cellular & Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06520-8082, USA.
Tiffany V LinDepartments of Neurosurgery, and Cellular & Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06520-8082, USA.
Xuan GongDepartment of Neurosurgery, Xiangya Hospital, Central South University, 85 Xiangya Street, Changsha, 410008, China; Departments of Neurosurgery, and Cellular & Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06520-8082, USA.
Longbo ZhangDepartment of Neurosurgery, Xiangya Hospital, Central South University, 85 Xiangya Street, Changsha, 410008, China; Departments of Neurosurgery, and Cellular & Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06520-8082, USA.
Gi Bum KimBoard of Governors Regenerative Medicine Institute, Center for Neural Science and Medicine, and Department of Biomedical Science Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA.
Matthew R SarkisianDepartment of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, FL, 32610, USA.
Joshua J BreunigBoard of Governors Regenerative Medicine Institute, Center for Neural Science and Medicine, and Department of Biomedical Science Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA; Department of Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, 90095, USA.
Angelique BordeyDepartment of Neurosurgery, Xiangya Hospital, Central South University, 85 Xiangya Street, Changsha, 410008, China; Departments of Neurosurgery, and Cellular & Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06520-8082, USA. Electronic address: angelique.bordey@yale.edu.
Central South University · CNCedars-Sinai Medical Center · USYale University · USUniversity of Florida · US

Funding

Integrated Interdisciplinary, inter-university PhD Program Computational Biology (T32)T32EB009403 · NIBIB · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BAHAR, IVET, BAR-JOSEPH, ZIV · 2009 to 2023
$4.4M
TSC-mTOR on neuron developmentR01NS086329 · NINDS · YALE UNIVERSITY · PI BORDEY, ANGELIQUE · 2013 to 2017
$1.8M
Filamin A in TSCR01NS093704 · NINDS · YALE UNIVERSITY · PI BORDEY, ANGELIQUE, CALDERWOOD, DAVID A · 2016 to 2020
$1.8M
NIBIB NIH HHS T32 EB009403NINDS NIH HHS R01 NS086329NINDS NIH HHS R01 NS093704
6 · The paper itself

Abstract

We combined translating ribosome affinity purification (TRAP) with in utero electroporation (IUE), called iTRAP to identify the molecular profile of specific neuronal populations during neonatal development without the need for viral approaches and FACS sorting. We electroporated a plasmid encoding EGFP-tagged ribosomal protein L10a at embryonic day (E) 14-15 to target layer 2-4 cortical neurons of the somatosensory cortex. At three postnatal (P) ages-P0, P7, and P14-when morphogenesis occurs and synapses are forming, TRAP and molecular profiling was performed from electroporated regions. We found that ribosome bound (Ribo)-mRNAs from ∼7300 genes were significantly altered over time and included classical neuronal genes known to decrease (e.g., Tbr1, Dcx) or increase (e.g., Eno2, Camk2a, Syn1) as neurons mature. This approach led to the identification of specific developmental patterns for Ribo-mRNAs not previously reported to be developmentally regulated in neurons, providing rationale for future examination of their role in selective biological processes. These include upregulation of Lynx1, Nrn1, Cntnap1 over time; downregulation of St8sia2 and Draxin; and bidirectional changes to Fkbp1b. iTRAP is a versatile approach that allows researchers to easily assess the molecular profile of specific neuronal populations in selective brain regions under various conditions, including overexpression and knockdown of target genes, and in disease settings.

Indexed as

Protein BiosynthesisAnimalsBrainDisks Large Homolog 4 ProteinDoublecortin ProteinElectroporationEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMaleMiceOligonucleotide Array Sequence AnalysisPyramidal CellsRecombinant Fusion ProteinsDcx protein, mouseDisks Large Homolog 4 ProteinDlg4 protein, mouseDoublecortin ProteinGreen Fluorescent ProteinsRecombinant Fusion ProteinsRibosomal ProteinsRNA, MessengerRpl10 protein, mouseSynapsinsCorticogenesisGlutamatergic neuronsMigrationSpineTRAP

Identifiers

PMID29857015
PMCPMC6265126
OpenAlexW2806357750

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.