Evidence map›Paper›PMID 29856812›Full record

ArticlePloS one2018

Galactose-1-phosphate uridyltransferase (GalT), an in vivo-induced antigen of Actinobacillus pleuropneumoniae serovar 5b strain L20, provided immunoprotection against serovar 1 strain MS71.

Fei Zhang, Qin Zhao, Keji Quan, Zhuang Zhu, Yusheng Yang, Xintian Wen, Yung-Fu Chang, Xiaobo Huang, Rui Wu, Yiping Wen and 5 more

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Review of advanced research on swineFrontiers in immunology · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 2 countries.

Fei ZhangResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Qin ZhaoResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Keji QuanResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Zhuang ZhuResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Yusheng YangResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Xintian WenResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Yung-Fu ChangDepartment of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States of America.
Xiaobo HuangResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Rui WuResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Yiping WenResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Qigui YanResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Yong HuangResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Xiaoping MaResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Xinfeng HanResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Sanjie CaoResearch Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.ORCID 0000-0002-2743-4840
Sichuan Agricultural University · CNCornell University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

GALT is an important antigen of Actinobacillus pleuropneumoniae (APP), which was shown to provide partial protection against APP infection in a previous study in our lab. The main purpose of the present study is to investigate GALT induced cross-protection between different APP serotypes and elucidate key mechanisms of the immune response to GALT antigenic stimulation. Bioinformatic analysis demonstrated that galT is a highly conserved gene in APP, widely distributed across multiple pathogenic strains. Homologies between any two strains ranges from 78.9% to 100% regarding the galT locus. Indirect enzyme-linked immunosorbent assay (ELISA) confirmed that GALT specific antibodies could not be induced by inactivated APP L20 or MS71 whole cell bacterin preparations. A recombinant fusion GALT protein derived from APP L20, however has proven to be an effective cross-protective antigen against APP sevorar 1 MS71 (50%, 4/8) and APP sevorar 5b L20 (75%, 6/8). Histopathological examinations have confirmed that recombinant GALT vaccinated animals showed less severe pathological signs in lung tissues than negative controls after APP challenge. Immunohistochemical (IHC) analysis indicated that the infiltration of neutrophils in the negative group is significantly increased compared with that in the normal control (P<0.001) and that in surviving animals is decreased compared to the negative group. Anti-GALT antibodies were shown to mediate phagocytosis of neutrophils. After interaction with anti-GALT antibodies, survival rate of APP challenged vaccinated animals was significantly reduced (P<0.001). This study demonstrated that GALT is an effective cross-protective antigen, which could be used as a potential vaccine candidate against multiple APP serotypes.

Indexed as

Actinobacillus InfectionsActinobacillus pleuropneumoniaeAnimalsAntibodies, BacterialAntigens, BacterialBacterial ProteinsBacterial VaccinesConserved SequenceDrug Evaluation, PreclinicalEnzyme-Linked Immunosorbent AssayFemaleImmunization, SecondaryImmunoglobulin GLungMiceMice, Inbred BALB CAntibodies, BacterialAntigens, BacterialApxIVA protein, Actinobacillus pleuropneumoniaeBacterial ProteinsBacterial VaccinesImmunoglobulin GUDPglucose-Hexose-1-Phosphate Uridylyltransferase

Identifiers

PMID29856812
PMCPMC5983418
OpenAlexW2807012016

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.