ArticlePloS one2018
Incubation of human sperm with micelles made from glycerophospholipid mixtures increases sperm motility and resistance to oxidative stress.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Gamma-aminobutyric acid modulates buck semen quality and sperm preservation duration in vivo and in vitro.Journal of animal science · 2026Article
- Untargeted Metabolomic Identifies Potential Seasonal Biomarkers of Semen Quality in Duroc Boars.Biology · 2025Article
- Beneficial Effects of Resveratrol on Testicular Functions: Focus on Its Antioxidant Properties.Cells · 2025Review
- Liposome-based Freezing Medium Improves the Outcome of Mouse Prepubertal Testicular Tissue Cryopreservation.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Evaluation of the effect of lecithin and nanolecithin in repairing membrane damage, maintaining membrane integrity, and improving human sperm function in the freezing-thawing process.Journal of assisted reproduction and genetics · 2024Article
- Rethinking the application of nanoparticles in women's reproductive health and assisted reproduction.Nanomedicine (London, England) · 2024Review
- Review of the Role of Ferroptosis in Testicular Function.Nutrients · 2022Review
- Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa.Genes · 2022Article
- Review
- High-Risk HPV with Multiple Infections Promotes CYP2E1, Lipoperoxidation and Pro-Inflammatory Cytokines in Semen of Asymptomatic Infertile Men.Antioxidants (Basel, Switzerland) · 2022Article
- Perspectives on Potential Fatty Acid Modulations of Motility Associated Human Sperm Ion Channels.International journal of molecular sciences · 2022Review
- Fatty Acid Oxidation and Pro-Resolving Lipid Mediators Are Related to Male Infertility.Antioxidants (Basel, Switzerland) · 2022Article
- Review
- Vitamin E Delivery Systems Increase Resistance to Oxidative Stress in Red Deer Sperm Cells: Hydrogel and Nanoemulsion Carriers.Antioxidants (Basel, Switzerland) · 2021Article
- Review
- High-coverage targeted lipidomics revealed dramatic lipid compositional changes in asthenozoospermic spermatozoa and inverse correlation of ganglioside GM3 with sperm motility.Reproductive biology and endocrinology : RB&E · 2021Article
- Glycerophospholipids protect stallion spermatozoa from oxidative damageReproduction & fertility · 2021Article
- Mitochondrial Dysfunction and Oxidative Stress Caused by Cryopreservation in Reproductive Cells.Antioxidants (Basel, Switzerland) · 2021Review
- Metabolite Support of Long-Term Storage of Sperm in the Spermatheca of Honeybee (Frontiers in physiology · 2020Article
- Relevance of Fatty Acids to Sperm Maturation and Quality.Oxidative medicine and cellular longevity · 2020Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane integrity is essential in maintaining sperm viability, signaling, and motility, which are essential for fertilization. Sperm are highly susceptible to oxidative stress, as they are rich in sensitive polyunsaturated fatty acids (PUFA), and are unable to synthesize and repair many essential membrane constituents. Because of this, sperm cellular membranes are important targets of this process. Membrane Lipid Replacement (MLR) with glycerophospholipid mixtures (GPL) has been shown to ameliorate oxidative stress in cells, restore their cellular membranes, and prevent loss of function. Therefore, we tested the effects of MLR on sperm by tracking and monitoring GPL incorporation into their membrane systems and studying their effects on sperm motility and viability under different experimental conditions. Incubation of sperm with mixtures of exogenous, unoxidized GPL results in their incorporation into sperm membranes, as shown by the use of fluorescent dyes attached to GPL. The percent overall (total) sperm motility was increased from 52±2.5% to 68±1.34% after adding GPL to the incubation media, and overall sperm motility was recovered from 7±2% after H2O2 treatment to 58±2.5%)(n = 8, p<0.01) by the incorporation of GPL into sperm membranes. When sperm were exposed to H2O2, the mitochondrial inner membrane potential (MIMP), monitored using the MIMP tracker dye JC-1 in flow cytometry, diminished, whereas the addition of GPL prevented the decrease in MIMP. Confocal microscopy with Rhodamine-123 and JC-1 confirmed the mitochondrial localization of the dyes. We conclude that incubation of human sperm with glycerolphospholipids into the membranes of sperm improves sperm viability, motility, and resistance to oxidizing agents like H2O2. This suggests that human sperm might be useful to test innovative new treatments like MLR, since such treatments could improve fertility when it is adversely affected by increased oxidative stress.
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