Evidence map›Paper›PMID 29855703›Full record

ArticleDevelopment genes and evolution2018

UTR-specific knockdown of Distal-less and Sp8 leads to new phenotypic variants in the flour beetle Tribolium.

Susanne Thümecke, Reinhard Schröder

Abstract read
PubMed Publisher
In one paragraph

Article in Development genes and evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Susanne ThümeckeInstitut für Biowissenschaften, Universität Rostock, Albert-Einsteinstr. 3, 18059, Rostock, Germany.
Reinhard SchröderInstitut für Biowissenschaften, Universität Rostock, Albert-Einsteinstr. 3, 18059, Rostock, Germany. reinhard.schroeder@uni-rostock.de.ORCID 0000-0002-3483-1916
University of Rostock · DE

Funding

Deutsche Forschungsgemeinschaft BE 4850/1-1
6 · The paper itself

Abstract

RNA interference (RNAi)-mediated knockdown serves as an effective technique for the functional analysis of developmental genes that is well established in many organisms. In the beetle Tribolium castaneum, double-stranded RNA is applied by simple injection and distributes systemically within the tissue. Thus, systematic testing for RNAi specificity and efficiency is easily possible in this organism. Generally, the use of non-overlapping dsRNA fragments yielding qualitatively identical phenotypes is the method of choice to verify target-specific knockdown effects. Here, we show that UTR-specific RNAi results in different effects regarding quality, severity and penetrance when compared to RNAi fragments directed at the coding region. Furthermore, when using 3'UTR-specific dsRNA, we first describe the Distal-less

Indexed as

3' Untranslated RegionsAnimalsHomeodomain ProteinsInsect ProteinsPhenotypeRNA, Double-StrandedRNA InterferenceTranscription FactorsTribolium3' Untranslated Regionsdistal-less protein, insectHomeodomain ProteinsInsect ProteinsRNA, Double-StrandedTranscription FactorsAppendage formationDistal-lessNOF-specific RNAi effectsRNAi efficacySp8Tribolium castaneumUTR-specific RNAi

Identifiers

PMID29855703
OpenAlexW2806780329

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.