ArticleMicrobial cell factories2018
Module and individual domain deletions of NRPS to produce plipastatin derivatives in Bacillus subtilis.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Reprogramming the enduracidin NRPS assembly line via thioesterase domain relocation.Synthetic and systems biotechnology · 2027Article
- Biocontrol of mushroom crop mycoparasites by novel Bacillus velezensis strains.Applied microbiology and biotechnology · 2026Article
- Systems-level genomic and functional characterization of lipopeptide biosurfactant pathways in Bacillus albus MITWPUB5.World journal of microbiology & biotechnology · 2026Article
- Biocontrol of mushroom crop mycoparasites by novelbioRxiv : the preprint server for biology · 2026Article
- Lipopeptide Engineering: From Natural Origins to Rational Design Against Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2026Review
- Multiple active-sites editing of adenylation domains of srfA operon for constructing diverse surfactin lipopeptide.Communications biology · 2026Article
- Lysogenic control of Bacillus subtilis morphology and fitness by Spbetavirus phi3T.Communications biology · 2025Article
- Genome-Guided Metabolomic Profiling of Peptaibol-ProducingInternational journal of molecular sciences · 2025Article
- Advances, opportunities, and challenges in methods for interrogating the structure activity relationships of natural products.Natural product reports · 2024Review
- Improving Surfactin Production inMicroorganisms · 2024Article
- Structural Diversity of the Lipopeptide Biosurfactant Produced by a Newly Isolated Strain,ACS omega · 2023Article
- Article
- Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced bySynthetic and systems biotechnology · 2022Article
- The Role of Epigenetic Modifications in Human Cancers and the Use of Natural Compounds as Epidrugs: Mechanistic Pathways and Pharmacodynamic Actions.Biomolecules · 2022Review
- Defining the Expression, Production, and Signaling Roles of Specialized Metabolites during Bacillus subtilis Differentiation.Journal of bacteriology · 2021Article
- Expression of degQ gene and its effect on lipopeptide production as well as formation of secretory proteases in Bacillus subtilis strains.MicrobiologyOpen · 2021Article
- Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis.Microbial cell factories · 2020Article
- Control of the polymyxin analog ratio by domain swapping in the nonribosomal peptide synthetase of Paenibacillus polymyxa.Journal of industrial microbiology & biotechnology · 2020Article
- Review
- Pass-back chain extension expands multimodular assembly line biosynthesis.Nature chemical biology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPlipastatin, an antifungal lipopeptide, is synthesized by a non-ribosomal peptide synthetase (NRPS) in Bacillus subtilis. However, little information is available on the combinatorial biosynthesis strategies applied in plipastatin biosynthetic pathway. In this study, we applied module or individual domain deletion strategies to engineer the plipastatin biosynthetic pathway, and investigated the effect of deletions on the plipastatin assembly line, as well as revealed the synthetic patterns of novel lipopeptides.
resultsModule deletion inactivated the entire enzyme complex, whereas individual domain (A/T domain) deletion within module 7 truncated the assembly line, resulting in truncated linear hexapeptides (C
conclusionsNotably, a unique module-skipping process occurred following deletion of the A
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Registered trials
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