Reconstruction of an SSR-based Magnaporthe oryzae physical map to locate avirulence gene AvrPi12.

Tonghui Li, Jianqiang Wen, Yaling Zhang, James Correll, Ling Wang, Qinghua Pan

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Article in BMC microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed

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2 citing papers in PubMed.

Arms and ammunitions: effectors at the interface of rice and it's pathogens and pests.Rice (New York, N.Y.) · 2021
Review
Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia.Scientific reports · 2021
Article

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6 authors.

Tonghui LiState Key laboratory for Conservation and Utilization of Subtropic Agrobioresurces, Guangdong Provincial Key Laboratory for Crop Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.

Jianqiang WenState Key laboratory for Conservation and Utilization of Subtropic Agrobioresurces, Guangdong Provincial Key Laboratory for Crop Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.

Yaling ZhangState Key laboratory for Conservation and Utilization of Subtropic Agrobioresurces, Guangdong Provincial Key Laboratory for Crop Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.

James CorrellDepartment of Plant Pathology, University of Arkansas, Fayetteville, AR, 72701, USA.

Ling WangState Key laboratory for Conservation and Utilization of Subtropic Agrobioresurces, Guangdong Provincial Key Laboratory for Crop Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.

Qinghua PanState Key laboratory for Conservation and Utilization of Subtropic Agrobioresurces, Guangdong Provincial Key Laboratory for Crop Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. panqh@scau.edu.cn.ORCID 0000-0002-0767-4089

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPathogen avirulence (Avr) genes can evolve rapidly when challenged by the widespread deployment of host genes for resistance. They can be effectively isolated by positional cloning provided a robust and well-populated genetic map is available.

resultsAn updated, SSR-based physical map of the rice blast pathogen Magnaporthe oryzae (Mo) has been constructed based on 116 of the 120 SSRs used to assemble the last map, along with 18 newly developed ones. A comparison between the two versions of the map has revealed an altered marker content and order within most of the Mo chromosomes. The avirulence gene AvrPi12 was mapped in a population of 219 progeny derived from a cross between the two Mo isolates CHL42 and CHL357. A bulked segregant analysis indicated that the gene was located on chromosome 6, a conclusion borne out by an analysis of the pattern of segregation shown by individual isolates. Six additional PCR-based markers were developed to improve the map resolution in the key region. AvrPi12 was finally located within the sub-telomeric region of chromosome 6, distal to the SSR locus LSM6-5.

conclusionsThe improved SSR-based linkage map should be useful as a platform for gene mapping and isolation in Mo. It was used to establish the location of AvrPi12, thereby providing a starting point for its positional cloning.

Indexed as

Chromosome SegregationFungal ProteinsMagnaportheMicrosatellite RepeatsOryzaPhysical Chromosome MappingPlant DiseasesFungal ProteinsAvirulence geneAvrPi12Genetic and physical mappingMagnaporthe oryzaeSSR physical map

Identifiers

PMID29855268

DOI10.1186/s12866-018-1192-x

PMCPMC5984427

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