ArticleBMC microbiology2018
A novel PBP3 substitution in Haemophilus influenzae confers reduced aminopenicillin susceptibility.
Article in BMC microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Evolution of beta-lactam resistance causes fitness reductions and several cases of collateral sensitivities in the human pathogenAntimicrobial agents and chemotherapy · 2025Article
- Genomic insight into the high-risk hypervirulent multidrug resistant enteroaggregative-hemorrhagicGene reports · 2025Article
- Genomic profiling of cefotaxime-resistantFrontiers in microbiology · 2025Article
- Comparative genomic study of non-typeableiScience · 2024Article
- Molecular Epidemiology and Antibiotic Resistance Analysis of Non-TypeableAntibiotics (Basel, Switzerland) · 2023Article
- Non-typeableFrontiers in cellular and infection microbiology · 2023Article
- β-lactam Resistance inPathogens (Basel, Switzerland) · 2021Review
- Characterization ofLife (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIdentification and characterization of non-typeable Haemophilus influenzae (NTHi) with reduced susceptibility to β-lactam antibiotics due to mutations in penicillin binding protein 3 (PBP3) is a clinical challenge. We analyzed a blood isolate, NTHi93-57485, that was categorized as aminopenicillin resistant but lacked key amino acid substitutions in PBP3 that have previously been associated with reduced aminopenicillin susceptibility. The significance of an alternative amino acid substitution (Y528H) in this isolate was examined.
resultsSite-directed mutagenesis of a β-lactam susceptible H. influenzae (NTHi3655) was performed to introduce the Y528H substitution into wild-type ftsI (encoding for PBP3). Disc diffusion screening and broth microdilution determination of MICs for β-lactam agents were done with the NTHi3655-PBP3
conclusionsIn parallel to the previously well-described PBP3-substitutions R517H and N526K, we demonstrate that Y528H confers reduced aminopenicillin susceptibility.
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