ArticleOxidative medicine and cellular longevity2018
L-Arginine Enhances Protein Synthesis by Phosphorylating mTOR (Thr 2446) in a Nitric Oxide-Dependent Manner in C2C12 Cells.
Article in Oxidative medicine and cellular longevity, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 69 citations in OpenAlex.
- Ginsenoside Rg3 attenuates fatigue by restoring autophagic homeostasis via the PI3K/AKT/mTOR pathway in ammonium chloride-induced C2C12 cells.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Potential role of L-citrulline in regulating exercise performance and muscle protein metabolism.NPJ science of food · 2026Review
- Early Growth and Serum Metabolic Profiling of One-Month-Old MSTN-Knockout Xinjiang Brown Cattle via CRISPR/Cas12Mix.Animals : an open access journal from MDPI · 2026Article
- Metabolomics and Transcriptomics Reveal the Effects of FermentedMetabolites · 2026Article
- Optimization of L-Arginine to Meet the Dietary Requirement of Juvenile Coho Salmon (Aquaculture nutrition · 2026Article
- Dietary nitrate supplementation mitigates age-related changes at the neuromuscular junction in mice.The Journal of physiology · 2026Article
- Spaceflight human optimization and performance: positioning astronauts in an operational framework to promote health, fitness, and mission readiness.Frontiers in physiology · 2026Review
- Arginine amino acid, nano particles of zinc oxide, and stock density: Effect on growth performance, intestinal morphology, blood indices, and meat quality in broiler chickens.Poultry science · 2026Article
- Metabolic signatures in osteoporotic cancellous bone: a comprehensive dual-platform metabolomic analysis.BMC musculoskeletal disorders · 2025Article
- Analysis of the Stimulative Effect of Arginine on Translation Initiation of Protein Synthesis in Skeletal Muscle.Nutrients · 2025Article
- Association between dietary intake of protein and amino acids and sarcopenia: a cross-sectional study.PloS one · 2025Article
- Nitric oxide participates in sucrose-TOR signaling during meristem activation in Arabidopsis thaliana.Planta · 2024Article
- Review
- Article
- Post-operative protein supplementation following orthopaedic surgery: A systematic review.Sports medicine and health science · 2024Review
- Effects ofFrontiers in microbiology · 2024Article
- Micelle encapsulated curcumin and piperine-laden 3D printed calcium phosphate scaffolds enhance in vitro biological properties.Colloids and surfaces. B, Biointerfaces · 2023Article
- Human skeletal muscle tissue chip autonomous payload reveals changes in fiber type and metabolic gene expression due to spaceflight.NPJ microgravity · 2023Article
- Targeted metabolomic profiling as a tool for diagnostics of patients with non-small-cell lung cancer.Scientific reports · 2023Article
- Impacts of microgravity on amino acid metabolism during spaceflight.Experimental biology and medicine (Maywood, N.J.) · 2023Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Muscle atrophy may arise from many factors such as inactivity, malnutrition, and inflammation. In the present study, we investigated the stimulatory effect of nitric oxide (NO) on muscle protein synthesis. Primarily, C2C12 cells were supplied with extra L-arginine (L-Arg) in the culture media. L-Arg supplementation increased the activity of inducible nitric oxide synthase (iNOS), the rate of protein synthesis, and the phosphorylation of mTOR (Thr 2446) and p70S6K (Thr 389). L-NAME, an NOS inhibitor, decreased NO concentrations within cells and abolished the stimulatory effect of L-Arg on protein synthesis and the phosphorylation of mTOR and p70S6K. In contrast, SNP (sodium nitroprusside), an NO donor, increased NO concentrations, enhanced protein synthesis, and upregulated mTOR and p70S6K phosphorylation, regardless of L-NAME treatment. Blocking mTOR with rapamycin abolished the stimulatory effect of both L-Arg and SNP on protein synthesis and p70S6K phosphorylation. These results indicate that L-Arg stimulates protein synthesis via the activation of the mTOR (Thr 2446)/p70S6K signaling pathway in an NO-dependent manner.
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