Evidence map›Paper›PMID 29853554›Full record

ArticleScience (New York, N.Y.)2018

Programming self-organizing multicellular structures with synthetic cell-cell signaling.

Satoshi Toda, Lucas R Blauch, Sindy K Y Tang, Leonardo Morsut, Wendell A Lim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 220 papers.

0numbers the graph read from it
0cells of the map it votes in
220citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

220 citing papers in PubMed.

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160 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Satoshi TodaDepartment of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, and Center for Systems and Synthetic Biology, University of California, San Francisco, CA 94158, USA.ORCID 0000-0003-4997-9558
Lucas R BlauchDepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0003-1517-0662
Sindy K Y TangDepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0003-1465-2432
Leonardo MorsutDepartment of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, and Center for Systems and Synthetic Biology, University of California, San Francisco, CA 94158, USA. leonardo.morsut@med.usc.edu wendell.lim@ucsf.edu.ORCID 0000-0001-7049-3478
Wendell A LimDepartment of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, and Center for Systems and Synthetic Biology, University of California, San Francisco, CA 94158, USA. leonardo.morsut@med.usc.edu wendell.lim@ucsf.edu.ORCID 0000-0003-4052-8056

Funding

Rewiring Therapeutic Cells: User-Controlled T Cells and Stem CellsP50GM081879 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MARSHALL, WALLACE · 2010 to 2017
$19.0M
Graduate Training Program in BiotechnologyT32GM008412 · NIGMS · STANFORD UNIVERSITY · PI COCHRAN, JENNIFER R · 1991 to 2020
$5.5M
Engineering Synthetic Receptor Systems That Can Detect Specific Cell-Cell Contact R00EB021030 · NIBIB · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MORSUT, LEONARDO · 2017 to 2019
$716k
Engineering Synthetic Receptor Systems That Can Detect Specific Cell-Cell Contact SignalsK99EB021030 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MORSUT, LEONARDO · 2015 to 2016
$195k
Howard Hughes Medical InstituteNIBIB NIH HHS K99 EB021030NIBIB NIH HHS R00 EB021030NIGMS NIH HHS P50 GM081879NIGMS NIH HHS T32 GM008412
6 · The paper itself

Abstract

A common theme in the self-organization of multicellular tissues is the use of cell-cell signaling networks to induce morphological changes. We used the modular synNotch juxtacrine signaling platform to engineer artificial genetic programs in which specific cell-cell contacts induced changes in cadherin cell adhesion. Despite their simplicity, these minimal intercellular programs were sufficient to yield assemblies with hallmarks of natural developmental systems: robust self-organization into multidomain structures, well-choreographed sequential assembly, cell type divergence, symmetry breaking, and the capacity for regeneration upon injury. The ability of these networks to drive complex structure formation illustrates the power of interlinking cell signaling with cell sorting: Signal-induced spatial reorganization alters the local signals received by each cell, resulting in iterative cycles of cell fate branching. These results provide insights into the evolution of multicellularity and demonstrate the potential to engineer customized self-organizing tissues or materials.

Indexed as

Artificial CellsCell CommunicationCellsMorphogenesisCell AdhesionCell EngineeringSignal TransductionSpheroids, Cellular

Identifiers

PMID29853554
PMCPMC6492944

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.