ArticleThe international journal of biochemistry & cell biology2018
SRRF: Universal live-cell super-resolution microscopy.
Article in The international journal of biochemistry & cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed.
- Synchronized Chromatin Reorganization as a Key Biomarker in Anesthetic Effects on Plants.Chemical & biomedical imaging · 2026Article
- ONE microscopy.Nature protocols · 2026Review
- Breaking the 100-nm resolution barrier with multiphoton microscopy using image scanning microscopy and optical fluctuation imaging.Journal of biomedical optics · 2026Article
- High-speed volumetric single-molecule imaging using dual-wavelength light sheets and PSF-engineered enhanced biplane detection.bioRxiv : the preprint server for biology · 2026Article
- Monitoring Lysosomal Viscosity in Hyperglycemia in Association with CaChemical & biomedical imaging · 2026Article
- Cluster assembly dynamics drive fidelity of planar cell polarity polarization.Nature communications · 2026Article
- High-fidelity single-frame computational super-resolution using signal-preserving denoising-enabled deconvolution.Nature communications · 2026Article
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Local GPCR density tips the balance of μ-opioid receptor trafficking.bioRxiv : the preprint server for biology · 2026Article
- ARP2/3 protein complex regulates myoblast proliferation, morphology, migration, and fusion during embryonic chick skeletal myogenesis.In vitro cellular & developmental biology. Animal · 2026Article
- Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues.Nature protocols · 2026Review
- Srcap Chromatin Remodeler Is Required for Efficient Replication Dynamics in Mammalian Cells.International journal of molecular sciences · 2025Article
- Super-resolution algorithms for imaging FCS enhancement: A comparative study.Biophysical journal · 2025Article
- Resolution assessment of super-resolution microscopy imaging: structural and technical dependencies for cell biology.Cytotechnology · 2025Article
- Huntingtin reduction results in altered nuclear structure and heterochromatic instability.Human molecular genetics · 2025Article
- Comparative Evaluation of Combined Denoising and Resolution Enhancement Algorithms for Intravital Two-Photon Imaging of Organs.Biosensors · 2025Article
- One-step nanoscale expansion microscopy reveals individual protein shapes.Nature biotechnology · 2025Article
- Super-resolution radial fluctuations (SRRF): a versatile and accessible tool for live-cell nanoscopy.Histochemistry and cell biology · 2025Review
- Novel structure and composition of the unusually large germline determinant of the wasp Nasonia vitripennis.Molecular biology of the cell · 2025Article
- Effects of Sup35 overexpression on the formation, morphology, and physiological functions of intracellular Sup35 assemblies.Applied and environmental microbiology · 2025Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
Super-resolution microscopy techniques break the diffraction limit of conventional optical microscopy to achieve resolutions approaching tens of nanometres. The major advantage of such techniques is that they provide resolutions close to those obtainable with electron microscopy while maintaining the benefits of light microscopy such as a wide palette of high specificity molecular labels, straightforward sample preparation and live-cell compatibility. Despite this, the application of super-resolution microscopy to dynamic, living samples has thus far been limited and often requires specialised, complex hardware. Here we demonstrate how a novel analytical approach, Super-Resolution Radial Fluctuations (SRRF), is able to make live-cell super-resolution microscopy accessible to a wider range of researchers. We show its applicability to live samples expressing GFP using commercial confocal as well as laser- and LED-based widefield microscopes, with the latter achieving long-term timelapse imaging with minimal photobleaching.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.