ArticlePLoS genetics2018
TPP riboswitch-dependent regulation of an ancient thiamin transporter in Candida.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
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- Whole cell affinity for 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in the marine bacterium Candidatus Pelagibacter st. HTCC7211 explains marine dissolved HMP concentrations.Environmental microbiology reports · 2024Article
- Discovering riboswitches: the past and the future.Trends in biochemical sciences · 2023Review
- Thiamine metabolism genes in diatoms are not regulated by thiamine despite the presence of predicted riboswitches.The New phytologist · 2022Article
- Riboswitch RSApplied and environmental microbiology · 2022Article
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- Aptamers, Riboswitches, and Ribozymes inLife (Basel, Switzerland) · 2021Review
- Riboswitches as Drug Targets for Antibiotics.Antibiotics (Basel, Switzerland) · 2021Review
- Origin, conservation, and loss of alternative splicing events that diversify the proteome in Saccharomycotina budding yeasts.RNA (New York, N.Y.) · 2020Article
- Intrinsic Regulatory Role of RNA Structural Arrangement in Alternative Splicing Control.International journal of molecular sciences · 2020Review
- Multilayered horizontal operon transfers from bacteria reconstruct a thiamine salvage pathway in yeasts.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Transcriptional Profiling of Stem Cells: Moving from Descriptive to Predictive Paradigms.Stem cell reports · 2019Review
- Genome sequence of the opportunistic human pathogen Magnusiomyces capitatus.Current genetics · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Riboswitches are non-coding RNA molecules that regulate gene expression by binding to specific ligands. They are primarily found in bacteria. However, one riboswitch type, the thiamin pyrophosphate (TPP) riboswitch, has also been described in some plants, marine protists and fungi. We find that riboswitches are widespread in the budding yeasts (Saccharomycotina), and they are most common in homologs of DUR31, originally described as a spermidine transporter. We show that DUR31 (an ortholog of N. crassa gene NCU01977) encodes a thiamin transporter in Candida species. Using an RFP/riboswitch expression system, we show that the functional elements of the riboswitch are contained within the native intron of DUR31 from Candida parapsilosis, and that the riboswitch regulates splicing in a thiamin-dependent manner when RFP is constitutively expressed. The DUR31 gene has been lost from Saccharomyces, and may have been displaced by an alternative thiamin transporter. TPP riboswitches are also present in other putative transporters in yeasts and filamentous fungi. However, they are rare in thiamin biosynthesis genes THI4 and THI5 in the Saccharomycotina, and have been lost from all genes in the sequenced species in the family Saccharomycetaceae, including S. cerevisiae.
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Registered trials
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