ArticlePloS one2018
Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Viable vs. Nonviable Microbiota Evaluation of the Hair Follicle and Scalp Microbiome.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Rapid Bacteria Identification and Antimicrobial Susceptibility Testing via 5-Min Fluorescence In Situ Hybridization.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Peptide Nucleic Acid-Fluorescence In Situ Hybridization (PNA-FISH) for the Detection and Spatial Location of Microorganisms.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Advanced Immunolabeling Method for Optical Volumetric Imaging Reveals Dystrophic Neurites of Dopaminergic Neurons in Alzheimer's Disease Mouse Brain.Molecular neurobiology · 2024Article
- Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Advances and Challenges in FluorescenceFrontiers in microbiology · 2022Article
- Article
- Spatiotemporal evaporating droplet dynamics on fomites enhances long term bacterial pathogenesis.Communications biology · 2021Article
- Combination of Direct Viable Count and Fluorescent In Situ Hybridization (DVC-FISH) as a Potential Method for Identifying ViableFoods (Basel, Switzerland) · 2021Article
- An Introduction to Fluorescence in situ Hybridization in Microorganisms.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A New PNA-FISH Probe TargetingFrontiers in cellular and infection microbiology · 2021Article
- Development of Fluorescence In Situ Hybridization as a Rapid, Accurate Method for Detecting Coliforms in Water Samples.Biosensors · 2020Article
- A novel cell permeability assay for macromolecules.BMC molecular and cell biology · 2020Article
- Article
- Cell Wall Anchoring of a Bacterial Chitosanase inInternational journal of molecular sciences · 2020Article
- Urban Particles Elevated Streptococcus pneumoniae Biofilms, Colonization of the Human Middle Ear Epithelial Cells, Mouse Nasopharynx and Transit to the Middle Ear and Lungs.Scientific reports · 2020Article
- Asian Sand Dust Particles Increased Pneumococcal Biofilm FormationFrontiers in genetics · 2020Article
- Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely- used technique in microbiology. The first step of FISH-fixation/permeabilization-is crucial to the outcome of the method. This work aimed to systematically evaluate fixation/permeabilization protocols employing ethanol, triton X-100 and lysozyme in conjugation with paraformaldehyde for Peptide Nucleic Acid (PNA)-FISH. Response surface methodology was used to optimize these protocols for Gram-negative (Escherichia coli and Pseudomonas fluorescens) and Gram-positive species (Listeria innocua, Staphylococcus epidermidis and Bacillus cereus). In general, the optimal PNA-FISH fluorescent outcome in Gram-positive bacteria was obtained employing harsher permeabilization conditions when compared to Gram-negative optimal protocols. The observed differences arise from the intrinsic cell envelope properties of each species and the ability of the fixation/permeabilization compounds to effectively increase the permeability of these structures while maintaining structural integrity. Ultimately, the combination of paraformaldehyde and ethanol proved to have significantly superior performance for all tested bacteria, especially for Gram-positive species (p<0.05).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.