ArticleMolecular biology of the cell2018
An isolated CLASP TOG domain suppresses microtubule catastrophe and promotes rescue.
Article in Molecular biology of the cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Kinetochore clustering is mediated by Mps1 phosphorylation of conserved MELT motifs in Stu1.The Journal of cell biology · 2026Article
- CLASP2 promotes repair of kinesin-1 damage to the microtubule lattice.bioRxiv : the preprint server for biology · 2026Article
- Kinetochore clustering is mediated by Mps1 phosphorylation of conserved MELT motifs in Stu1.bioRxiv : the preprint server for biology · 2026Article
- APC orchestrates microtubule dynamics by acting as a positive regulator of KIF2A and a negative regulator of CLASPs.Cell insight · 2025Article
- Poxvirus A51R proteins regulate microtubule stability and antagonize a cell-intrinsic antiviral response.Cell reports · 2024Article
- CLASPs stabilize the pre-catastrophe intermediate state between microtubule growth and shrinkage.The Journal of cell biology · 2023Article
- The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network.The EMBO journal · 2023Article
- Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- CLASP2 recognizes tubulins exposed at the microtubule plus-end in a nucleotide state-sensitive manner.Science advances · 2023Article
- Beyond the GTP-cap: Elucidating the molecular mechanisms of microtubule catastrophe.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- MAPping tubulin mutations.Frontiers in cell and developmental biology · 2023Review
- Quantification of microtubule stutters: dynamic instability behaviors that are strongly associated with catastrophe.Molecular biology of the cell · 2022Article
- The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules.eLife · 2022Article
- Reconstituting Microtubules: A Decades-Long Effort From Building Block Identification to the Generation of Recombinant α/β-Tubulin.Frontiers in cell and developmental biology · 2022Review
- Regulation of microtubule dynamics, mechanics and function through the growing tip.Nature reviews. Molecular cell biology · 2021Review
- TH588 and Low-Dose Nocodazole Impair Chromosome Congression by Suppressing Microtubule Turnover within the Mitotic Spindle.Cancers · 2021Article
- Differential regulation of single microtubules and bundles by a three-protein module.Nature chemical biology · 2021Article
- Impact of the 'tubulin economy' on the formation and function of the microtubule cytoskeleton.Current opinion in cell biology · 2021Review
- A Multipronged Approach Establishes Covalent Modification of β-Tubulin as the Mode of Action of Benzamide Anti-cancer Toxins.Journal of medicinal chemistry · 2020Article
- A cryptic tubulin-binding domain links MEKK1 to curved tubulin protomers.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Microtubules are heavily regulated dynamic polymers of αβ-tubulin that are required for proper chromosome segregation and organization of the cytoplasm. Polymerases in the XMAP215 family use arrayed TOG domains to promote faster microtubule elongation. Regulatory factors in the cytoplasmic linker associated protein (CLASP) family that reduce catastrophe and/or increase rescue also contain arrayed TOGs, but how CLASP TOGs contribute to activity is poorly understood. Here, using Saccharomyces cerevisiae Stu1 as a model CLASP, we report structural, biochemical, and reconstitution studies that clarify functional properties of CLASP TOGs. The two TOGs in Stu1 have very different tubulin-binding properties: TOG2 binds to both unpolymerized and polymerized tubulin, and TOG1 binds very weakly to either. The structure of Stu1-TOG2 reveals a CLASP-specific residue that likely confers distinctive tubulin-binding properties. The isolated TOG2 domain strongly suppresses microtubule catastrophe and increases microtubule rescue in vitro, contradicting the expectation that regulatory activity requires an array of TOGs. Single point mutations on the tubulin-binding surface of TOG2 ablate its anti-catastrophe and rescue activity in vitro, and Stu1 function in cells. Revealing that an isolated CLASP TOG can regulate polymerization dynamics without being part of an array provides insight into the mechanism of CLASPs and diversifies the understanding of TOG function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.