Evidence map›Paper›PMID 29851463›Full record

ArticleACS chemical biology2018

Subcellular Protein Labeling by a Spatially Restricted Arylamine N-Acetyltransferase.

Fleur Kleinpenning, Selma Eising, Tim Berkenbosch, Veronica Garzero, Judith M Schaart, Kimberly M Bonger

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Fleur KleinpenningDepartment of Biomolecular Chemistry, Institute of Molecules and Materials , Radboud University , Nijmegen , Heyendaalseweg 135 , 6525 AJ , The Netherlands.
Selma EisingDepartment of Biomolecular Chemistry, Institute of Molecules and Materials , Radboud University , Nijmegen , Heyendaalseweg 135 , 6525 AJ , The Netherlands.
Tim BerkenboschDepartment of Biomolecular Chemistry, Institute of Molecules and Materials , Radboud University , Nijmegen , Heyendaalseweg 135 , 6525 AJ , The Netherlands.
Veronica GarzeroDepartment of Biomolecular Chemistry, Institute of Molecules and Materials , Radboud University , Nijmegen , Heyendaalseweg 135 , 6525 AJ , The Netherlands.
Judith M SchaartDepartment of Biomolecular Chemistry, Institute of Molecules and Materials , Radboud University , Nijmegen , Heyendaalseweg 135 , 6525 AJ , The Netherlands.
Kimberly M BongerDepartment of Biomolecular Chemistry, Institute of Molecules and Materials , Radboud University , Nijmegen , Heyendaalseweg 135 , 6525 AJ , The Netherlands.ORCID 0000-0001-9498-2620
Radboud University Nijmegen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mapping proteins at a specific subcellular location is essential to gaining detailed insight on local protein dynamics. We have developed an enzymatic strategy to label proteins on a subcellular level using arylamine N-acetyltransferase (NAT). The NAT enzyme activates an arylhydroxamic acid functionality into a nitrenium ion that reacts fast, covalently, and under neutral conditions with nucleophilic residues of neighboring proteins. The electron density on the aromatic ring proved important for probe activation as strong labeling was only observed with an arylhydroxamic acid bearing an electron donating substituent. We further demonstrate that, using this electron rich arylhydroxamic acid, clear labeling was achieved on a subcellular level in living cells that were transfected with a genetically targeted NAT to the nucleus or the cytosol.

Indexed as

AcetanilidesArylamine N-AcetyltransferaseCell NucleusCytosolHEK293 CellsHumansHydroxamic AcidsIsoenzymesMolecular ProbesNuclear Localization SignalsProteinsProteomicsAcetanilidesArylamine N-AcetyltransferaseHydroxamic AcidsIsoenzymesMolecular ProbesN-acetyltransferase 1NAT2 protein, humanNuclear Localization SignalsProteins

Identifiers

PMID29851463
PMCPMC6143282
OpenAlexW2807089068

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.