ArticleApplied microbiology and biotechnology2018
Characterization of the caprolactam degradation pathway in Pseudomonas jessenii using mass spectrometry-based proteomics.
Article in Applied microbiology and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
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- Integrative Analysis of Nontargeted LC-HRMS and High-Throughput Metabarcoding Data for Aquatic Environmental Studies Using Combined Multivariate Statistical Approaches.Analytical chemistry · 2025Article
- Recycling and Degradation Pathways of Synthetic Textile Fibers such as Polyamide and Elastane.Global challenges (Hoboken, NJ) · 2025Review
- Upcycling of polyamides through chemical hydrolysis and engineered Pseudomonas putida.Nature microbiology · 2025Article
- Mild hydrolysis of chemically stable valerolactams by a biocatalytic ATP-dependent system fueled by metaphosphate.Green chemistry : an international journal and green chemistry resource : GC · 2024Article
- A metagenomic catalog for exploring the plastizymes landscape covering taxa, genes, and proteins.Scientific reports · 2023Article
- Evaluating non-targeted analysis methods for chemical characterization of organic contaminants in different matrices to estimate children's exposure.Journal of exposure science & environmental epidemiology · 2023Article
- Characterization of a NewMicroorganisms · 2023Article
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- Quantitative proteomic analysis of the microbial degradation of 3-aminobenzoic acid by Comamonas sp. QT12.Scientific reports · 2022Article
- In silico prediction of the enzymes involved in the degradation of the herbicide molinate by Gulosibacter molinativorax ON4Scientific reports · 2022Article
- Catalytic and structural properties of ATP-dependent caprolactamase from Pseudomonas jessenii.Proteins · 2021Article
- Label-Free Quantitative Proteomic Analysis of the Global Response to Indole-3-Acetic Acid in Newly IsolatedFrontiers in microbiology · 2021Article
- Omics-driven identification and elimination of valerolactam catabolism inMetabolic engineering communications · 2019Article
- An Arthrobacter citreus strain suitable for degrading ε-caprolactam in polyamide waste and accumulation of glutamic acid.AMB Express · 2019Article
- Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine Degradation.Current microbiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Some bacterial cultures are capable of growth on caprolactam as sole carbon and nitrogen source, but the enzymes of the catabolic pathway have not been described. We isolated a caprolactam-degrading strain of Pseudomonas jessenii from soil and identified proteins and genes putatively involved in caprolactam metabolism using quantitative mass spectrometry-based proteomics. This led to the discovery of a caprolactamase and an aminotransferase that are involved in the initial steps of caprolactam conversion. Additionally, various proteins were identified that likely are involved in later steps of the pathway. The caprolactamase consists of two subunits and demonstrated high sequence identity to the 5-oxoprolinases. Escherichia coli cells expressing this caprolactamase did not convert 5-oxoproline but were able to hydrolyze caprolactam to form 6-aminocaproic acid in an ATP-dependent manner. Characterization of the aminotransferase revealed that the enzyme deaminates 6-aminocaproic acid to produce 6-oxohexanoate with pyruvate as amino acceptor. The amino acid sequence of the aminotransferase showed high similarity to subgroup II ω-aminotransferases of the PLP-fold type I proteins. Finally, analyses of the genome sequence revealed the presence of a caprolactam catabolism gene cluster comprising a set of genes involved in the conversion of caprolactam to adipate.
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