ArticleNucleic acids research2018
The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- A competitive regulatory mechanism of the Chd1 remodeler is integral to distorting nucleosomal DNA.Nature structural & molecular biology · 2025Article
- Solution conformational differences between conventional and CENP-A nucleosomes are accentuated by reversible deformation under high pressure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Regulation of DNA translocation of chromatin remodeler enzyme Chd1 by exit DNA unwrapping.Life metabolism · 2025Article
- CHD1 dysregulation in cancer: bridging chromatin instability, therapy resistance, and immune evasion.Molecular biology reports · 2025Review
- Folate intake and colorectal cancer risk according to genetic subtypes defined by targeted tumor sequencing.The American journal of clinical nutrition · 2024Article
- Article
- Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.Nature communications · 2024Article
- Sample-minimizing co-flow cell for time-resolved pump-probe X-ray solution scattering.Journal of synchrotron radiation · 2023Article
- Coordinated DNA and histone dynamics drive accurate histone H2A.Z exchange.Science advances · 2022Article
- Contrast variation SAXS: Sample preparation protocols, experimental procedures, and data analysis.Methods in enzymology · 2022Article
- Sentinels of chromatin: chromodomain helicase DNA-binding proteins in development and disease.Genes & development · 2021Review
- Collaboration through chromatin: motors of transcription and chromatin structure.Journal of molecular biology · 2021Review
- Torsional stress can regulate the unwrapping of two outer half superhelical turns of nucleosomal DNA.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Chd1 chromatin remodeler forms long-lived complexes with nucleosomes in the presence of ADP·BeFThe Journal of biological chemistry · 2019Article
- Structure of the primed state of the ATPase domain of chromatin remodeling factor ISWI bound to the nucleosome.Nucleic acids research · 2019Article
- Direct observation of coordinated DNA movements on the nucleosome during chromatin remodelling.Nature communications · 2019Article
- Nucleosome structure and dynamics are coming of age.Nature structural & molecular biology · 2019Review
- Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome.eLife · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Chromatin remodelers are ATP-dependent motors that reorganize DNA packaging by disrupting canonical histone-DNA contacts within the nucleosome. Here, we show that the Chd1 chromatin remodeler stimulates DNA unwrapping from the edge of the nucleosome in a nucleotide-dependent and DNA sequence-sensitive fashion. Nucleosome binding, monitored by stopped flow, was complex and sensitive to nucleotide, with AMP-PNP promoting faster binding than ADP·BeF3-. Nucleosome unwrapping by Chd1, examined by bulk FRET, occurred in the presence and absence of nucleotide and did not require the Chd1 DNA-binding domain. In AMP-PNP conditions, Chd1 unwrapped one side of the Widom 601 DNA more easily than the other, consistent with previous observations of 601 asymmetry and indicating that Chd1 amplifies intrinsic sequence properties of nucleosomal DNA. Using small angle X-ray scattering (SAXS) with contrast variation, we found distinct DNA conformations depending on the nucleotide analog bound to Chd1: with AMP-PNP, DNA primarily unwrapped in-plane with the nucleosomal disk, whereas with ADP·BeF3-, a significant fraction showed distinctive out-of-plane unwrapping as well. Taken together, our findings show tight coupling between entry/exit DNA of the nucleosome and the Chd1 ATPase motor, suggesting that dynamic nucleosome unwrapping is coupled to nucleosome binding and remodeling by Chd1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.