ArticleBioMed research international2018
Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes.
Article in BioMed research international, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Galectin-12 in the Regulation of Sebocyte Proliferation, Lipid Metabolism, and Immune Responses.Biomolecules · 2025Review
- Emodin exhibits anti-acne potential by inhibiting cell growth, lipogenesis, and inflammation in human SZ95 sebocytes.Scientific reports · 2023Article
- The World's First Acne Dysbiosis-like Model of Human 3D Ex Vivo Sebaceous Gland Colonized withMicroorganisms · 2023Article
- The Osteogenic Differentiation of Human Dental Pulp Stem Cells through G0/G1 Arrest and the p-ERK/Runx-2 Pathway by Sonic Vibration.International journal of molecular sciences · 2021Article
- Establishment and characterization of a canine sebaceous epithelial cell line derived from an eyelid mass.The Journal of veterinary medical science · 2020Article
- Biguanides Induce Acute de novo Lipogenesis in Human Primary Sebocytes.Clinical, cosmetic and investigational dermatology · 2020Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sebocyte differentiation is a continuous process, but its potential molecular mechanism remains unclear. We aimed to establish a novel sebocyte differentiation model using human primary sebocytes and to identify the expression profiles of differentiation-associated proteins. Primary human sebocytes were cultured on Sebomed medium supplemented with 2% serum for 7 days. Flow cytometry showed that S phase cells were decreased time-dependently, while G1 and subG1 (apoptosis) phase cells increased under serum starvation. Transmission electron microscopy and Oil Red O staining revealed a gradual increase of intracellular lipid accumulation. Expression of proliferation marker was diminished, while expression of differentiation, apoptosis, and lipogenic markers elevated gradually during 7-day culture. iTRAQ analysis identified 3582 expressed proteins in this differentiation model. Compared with day 0, number of differentially expressed proteins was 132, 54, 321, and 96 at days 1, 3, 5, and 7, respectively. Two overexpressed proteins (S100 calcium binding protein P and ferredoxin reductase) and 2 downexpressed proteins (adenosine deaminase and keratin 10) were further confirmed by Western blot and immunohistochemistry.
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