Evidence map›Paper›PMID 29849743›Full record

ArticlePlant methods2018

Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging.

Sadie J Burkhow, Nicole M Stephens, Yu Mei, Maria Emilia Dueñas, Daniel J Freppon, Geng Ding, Shea C Smith, Young-Jin Lee, Basil J Nikolau, Steven A Whitham and 1 more

Open access · goldAbstract read
In one paragraph

Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Sadie J Burkhow1The Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011-3111 USA.
Nicole M Stephens1The Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011-3111 USA.
Yu Mei3Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011 USA.
Maria Emilia Dueñas1The Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011-3111 USA.
Daniel J Freppon1The Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011-3111 USA.
Geng Ding4Department of Biochemistry Biophysics, and Molecular Biology, Center for Metabolic Biology, Iowa State University, Ames, IA 50011 USA.
Shea C Smith5Engineering Research Center for Biorenewable Chemicals, Iowa State University, Ames, IA 50011 USA.
Young-Jin Lee1The Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011-3111 USA.
Basil J Nikolau4Department of Biochemistry Biophysics, and Molecular Biology, Center for Metabolic Biology, Iowa State University, Ames, IA 50011 USA.
Steven A Whitham3Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011 USA.
Emily A Smith1The Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011-3111 USA.ORCID 0000-0001-7438-7808
Iowa State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundReverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study gene function. Currently, there are few tools to study the spatial dependence of the consequences of gene silencing at the cellular level.

resultsWe report the use of multimodal Raman and mass spectrometry imaging to study the cellular-level biochemical changes that occur from silencing the

conclusionsBoth Raman and mass spectrometry imaging show that the biochemical changes from FoMV-

Indexed as

Biochemical characterizationCarotenoidsFoxtail mosaic virusMass spectrometry imagingMosaic spatial patternPhytoene desaturaseRNA silencingSubcellular Raman imagingWhole-plant analysis

Identifiers

PMID29849743
PMCPMC5968576
OpenAlexW2805842382

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.