ArticleScientific reports2018
A robust ex vivo experimental platform for molecular-genetic dissection of adult human neocortical cell types and circuits.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 130 citations in OpenAlex.
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- DuBois-Reymond prize lecture 2024: Multi-neuron patch-clamp to uncover the neuronal and synaptic physiology in the human neocortex.Pflugers Archiv : European journal of physiology · 2026Article
- Intrinsic neuronal diversity as a substrate for cortical area specialization in primate vision.Nature communications · 2026Article
- Combined patch-clamp electrophysiology and single-cell genomic analysis reveal spiking tumor cells at the neocortical glioblastoma interface in humans.Neuro-oncology · 2026Article
- Mapping spatially organized molecular and genetic signatures of schizophrenia across multiple scales in human prefrontal cortex.bioRxiv : the preprint server for biology · 2026Article
- Inhibitory GABAergic Neuron Loss due to Oxidative Damage During Ex Vivo Acute Brain Slice Preparation Influences Genesis and Dynamics of Epileptiform Activity.Journal of neurochemistry · 2026Article
- Human Neocortical Glutamatergic Neurons Revealed Through Multimodal Profiling.bioRxiv : the preprint server for biology · 2026Article
- Live-cell physiology in human brain tissue culture-the potential, the challenges, and the lessons learned.Frontiers in cellular neuroscience · 2026Review
- The Freiburg framework for multimodalFrontiers in synaptic neuroscience · 2026Article
- Enhanced information processing in the human neocortex: cellular mechanisms and translational perspectives.Frontiers in synaptic neuroscience · 2026Review
- Microscale maps of bursting dynamics across human hippocampal slices from patients with epilepsy.Journal of neurophysiology · 2026Article
- The grand challenge in cellular neurophysiology.Frontiers in cellular neuroscience · 2026Article
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- CulturedBrain communications · 2026Article
- RNA-programmable cell-type monitoring and manipulation in the human cortex with CellREADR.Cell reports · 2025Article
- Divergent actions of physiological and pathological amyloid-β on synapses in live human brain slice cultures.Nature communications · 2025Article
- Pathological microcircuits and epileptiform events in patient hippocampal slices.bioRxiv : the preprint server for biology · 2025Article
- Ex vivo study of neuroinvasive and neurotropic viruses: what is current and what is next.FEMS microbiology reviews · 2025Review
- Exploring the singularity of human neurons: keep calm and carry on.Frontiers in synaptic neuroscience · 2025Review
- Elevated glioma-related cortical glutamateBrain communications · 2025Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
The powerful suite of available genetic tools is driving tremendous progress in understanding mouse brain cell types and circuits. However, the degree of conservation in human remains largely unknown in large part due to the lack of such tools and healthy tissue preparations. To close this gap, we describe a robust and stable adult human neurosurgically-derived ex vivo acute and cultured neocortical brain slice system optimized for rapid molecular-genetic manipulation. Surprisingly, acute human brain slices exhibited exceptional viability, and neuronal intrinsic membrane properties could be assayed for at least three days. Maintaining adult human slices in culture under sterile conditions further enabled the application of viral tools to drive rapid expression of exogenous transgenes. Widespread neuron-specific labeling was achieved as early as two days post infection with HSV-1 vectors, with virally-transduced neurons exhibiting membrane properties largely comparable to uninfected neurons over this short timeframe. Finally, we demonstrate the suitability of this culture paradigm for optical manipulation and monitoring of neuronal activity using genetically encoded probes, opening a path for applying modern molecular-genetic tools to study human brain circuit function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.