ArticleInvestigative ophthalmology & visual science2018
High-Resolution, Three-Dimensional Reconstruction of the Outflow Tract Demonstrates Segmental Differences in Cleared Eyes.
Article in Investigative ophthalmology & visual science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 8 citations in OpenAlex.
- Vascular regeneration and blood flow recovery in glaucoma.Frontiers in cell and developmental biology · 2026Review
- Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis.Journal of ophthalmology · 2026Review
- Light sheet fluorescence microscopy of cleared human eyes.Communications biology · 2023Article
- Long-Term Clinical Results of Trabectome Surgery in Turkish Patients with Primary Open Angle Glaucoma and Pseudoexfoliative Glaucoma.Beyoglu eye journal · 2023Article
- 'Intraoperative predictors for clinical outcomes after microinvasive glaucoma surgery".PloS one · 2023Article
- Valve-Like Outflow System Behavior With Motion Slowing in Glaucoma Eyes: Findings Using a Minimally Invasive Glaucoma Surgery-MIGS-Like Platform and Optical Coherence Tomography Imaging.Frontiers in medicine · 2022Article
- Aqueous outflow regulation - 21st century concepts.Progress in retinal and eye research · 2021Review
- Aqueous outflow imaging techniques and what they tell us about intraocular pressure regulation.Eye (London, England) · 2021Review
- Aqueous Angiography in Normal Canine Eyes.Translational vision science & technology · 2020Article
- Shear Stress in Schlemm's Canal as a Sensor of Intraocular Pressure.Scientific reports · 2020Article
- Minimally Invasive Glaucoma Surgery: What Do We Know? Where Should We Go?Translational vision science & technology · 2020Review
- The Effectiveness of First-Generation iStent Microbypass Implantation Depends on Initial Intraocular Pressure: 24-Month Follow-Up-Prospective Clinical Trial.Journal of ophthalmology · 2020Article
- 3D-Reconstruction of the human conventional outflow system by ribbon scanning confocal microscopy.PloS one · 2020Article
- Imaging Distal Aqueous Outflow Pathways in a Spontaneous Model of Congenital Glaucoma.Translational vision science & technology · 2019Article
- Outflow facility and extent of angle closure in a porcine model.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Article
- Five-year clinical outcomes of combined phacoemulsification and trabectome surgery at a single glaucoma center.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Article
- Neutrophils homing into the retina trigger pathology in early age-related macular degeneration.Communications biology · 2019Article
- Structure-Function Changes of the Porcine Distal Outflow Tract in Response to Nitric Oxide.Investigative ophthalmology & visual science · 2018Article
- Outflow enhancement by three different ab interno trabeculectomy procedures in a porcine anterior segment model.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2018Article
- Impact of aging on anterior segment morphology and aqueous humor dynamics in human Eyes: Advanced imaging and computational techniques.Biocybernetics and biomedical engineeringArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Purpose: The rate of conventional aqueous humor outflow is the highest nasally. We hypothesized that this is reflected in regionally different outflow structures and analyzed the entire limbus by high-resolution, full-thickness ribbon-scanning confocal microscopy (RSCM). Methods: We perfused pig eyes by anterior chamber cannulation with eight lectin-fluorophore conjugates, followed by optical clearance with benzyl alcohol benzyl benzoate (BABB). RSCM and advanced analysis software (Imaris) were used to reconstruct a three-dimensional (3D), whole-specimen rendering of the perilimbal outflow structures. We performed morphometric analyses of the outflow tract from the level of the trabecular meshwork (TM) to the scleral vascular plexus (SVP). Results: Except for pigmented structures, BABB cleared the entire eye. Rhodamine-conjugated Glycine max agglutinin (soybean [SBA]) labeled the outflow tract evenly and retained fluorescence for months. RSCM produced terabyte-sized files allowing for in silico dissection of outflow tract vessels at a high resolution and in 3D. Networks of interconnected lumens were traced from the TM to downstream drainage structures. The collector channel (CC) volumes were 10 times smaller than the receiving SVP vessels, the largest of which were in the inferior limbus. Proximal CC diameters were up to four times the size of distal diameters and more elliptical at their proximal ends. The largest CCs were found in the superonasal and inferonasal quadrants where the highest outflow occurs. Conclusion: RSCM of cleared eyes enabled high-resolution, volumetric analysis of the outflow tract. The proximal structures had greater diameters nasally, whereas the SVP was larger in the inferior limbus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.