ArticlePloS one2018
Roles of B739_1343 in iron acquisition and pathogenesis in Riemerella anatipestifer CH-1 and evaluation of the RA-CH-1ΔB739_1343 mutant as an attenuated vaccine.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Deciphering defensive mechanisms inFrontiers in immunology · 2026Review
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- Research Progress in the Development of Vaccines AgainstMicroorganisms · 2025Review
- Functional characterization of RhuB as a second TonB2-dependent hemin receptor inMicrobiology spectrum · 2024Article
- PMCNA_RS00975 activates NF-κB and ERK1/2 through TLR2 and contributes to the virulence ofFrontiers in cellular and infection microbiology · 2024Article
- Functional characterization of two TolC in the resistance to drugs and metals and in the virulence ofApplied and environmental microbiology · 2023Article
- In Silico Analysis and Immune Response of YaeT Protein Against Riemerella anatipestifer in Ducks.Applied biochemistry and biotechnology · 2023Article
- Functional Characterization of FeoAB in Iron Acquisition and Pathogenicity in Riemerella anatipestifer.Microbiology spectrum · 2023Article
- An Exposed Outer Membrane Hemin-Binding Protein Facilitates Hemin Transport by a TonB-Dependent Receptor in Riemerella anatipestifer.Applied and environmental microbiology · 2021Article
- Functional characterization of Fur in iron metabolism, oxidative stress resistance and virulence of Riemerella anatipestifer.Veterinary research · 2021Article
- Comparative genomics and metabolomics analysis of Riemerella anatipestifer strain CH-1 and CH-2.Scientific reports · 2021Article
- Application of TonB-Dependent Transporters in Vaccine Development of Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2020Review
- New Perspectives on Galleria mellonella Larvae as a Host Model Using Riemerella anatipestifer as a Proof of Concept.Infection and immunity · 2019Article
- Riemerella anatipestifer GldM is required for bacterial gliding motility, protein secretion, and virulence.Veterinary research · 2019Article
- Role of LptD in Resistance to Glutaraldehyde and Pathogenicity inFrontiers in microbiology · 2019Article
- DprA Is Essential for Natural Competence inFrontiers in genetics · 2019Article
- Article
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14 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Iron is one of the most important elements for bacterial survival and pathogenicity. The iron uptake mechanism of Riemerella anatipestifer (R. anatipestifer, RA), a major pathogen that causes septicemia and polyserositis in ducks, is largely unknown. Here, the functions of the putative TonB-dependent iron transporter of RA-CH-1, B739_1343, in iron utilization and pathogenicity were investigated. Under iron-starved conditions, the mutant strain RA-CH-1ΔB739_1343 exhibited more seriously impaired growth than the wild-type strain RA-CH-1, and the expression of B739_1343 in the mutant strain restored growth. qRT-PCR results showed that the transcription of B739_1343 was not regulated by iron conditions. In an animal model, the median lethal dose (LD50) of the mutant strain RA-CH-1ΔB739_1343 increased more than 104-fold (1.6×1012 CFU) compared to that of the wild-type strain RA-CH-1 (1.43×108 CFU). In a duck co-infection model, the mutant strain RA-CH-1ΔB739_1343 was outcompeted by the wild-type RA-CH-1 in the blood, liver and brain of infected ducks, indicating that B739_1343 is a virulence factor of RA-CH-1. Finally, immunization with live bacteria of the mutant strain RA-CH-1ΔB739_1343 protected 83.33% of ducks against a high-dose (100-fold LD50) challenge with the wild-type strain RA-CH-1, suggesting that the mutant strain RA-CH-1ΔB739_1343 could be further developed as a potential live attenuated vaccine candidate for the duck industry.
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