ArticlePloS one2018
Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- GH3 phylogenetic subgroups define divergent routes of auxin inactivation via aspartate and glutamine conjugation.Plant physiology · 2026Article
- The plant Golden Gate toolkit: compatibility, interoperability, and harmonized assembly in plant synthetic biology.Plant methods · 2026Review
- Root developmental zonation is independent of cell wall pH.Nature plants · 2026Article
- Insight into the genetic network governing long-stalked glandular trichome development in Nicotiana tabacum.Planta · 2025Article
- Assembling DNA Plasmids with the Multi-Kingdom (MK) Cloning System.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Golden Gate Cloning of MoClo Standard Parts.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Live Single-Cell Transcriptional Dynamics in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Characterization of the ZCTs, a subgroup of Cys2-His2 zinc finger transcription factors regulating alkaloid biosynthesis in Catharanthus roseus.Plant cell reports · 2024Article
- Posttranslational regulation of photosynthetic activity via the TOR kinase in plants.Science advances · 2024Article
- EngineeringFrontiers in plant science · 2024Article
- Greater than the sum of their parts: an overview of the AvrRps4 effector family.Frontiers in plant science · 2024Review
- Tailored biosynthesis of diosmin through reconstitution of the flavonoid pathway inFrontiers in plant science · 2024Article
- Functional Analysis of Plant Monosaccharide Transporters Using a Simple Growth Complementation Assay in Yeast.Bio-protocol · 2023Article
- An extensible vector toolkit and parts library for advanced engineering of plant genomes.The plant genome · 2023Article
- Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two.Plant methods · 2023Article
- Systematic Comparison of Plant Promoters inInternational journal of molecular sciences · 2022Article
- A User's Guide to Golden Gate Cloning Methods and Standards.ACS synthetic biology · 2022Review
- MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein-protein interactions.Plant methods · 2022Article
- Members of the ribosomal protein S6 (RPS6) family act as pro-viral factor for tomato spotted wilt orthotospovirus infectivity in Nicotiana benthamiana.Molecular plant pathology · 2022Article
- Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP).Methods in molecular biology (Clifton, N.J.) · 2022Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type IIs restriction endonucleases was recently developed, and underlies the Modular Cloning and GoldenBraid systems. In these systems, transcriptional units and/or multigene constructs are assembled from libraries of standardized building blocks, also referred to as phytobricks, in several hierarchical levels and by iterative Golden Gate reactions. Here, a toolkit containing further modules for the novel DNA assembly standards was developed. Intended for use with Modular Cloning, most modules are also compatible with GoldenBraid. Firstly, a collection of approximately 80 additional phytobricks is provided, comprising e.g. modules for inducible expression systems, promoters or epitope tags. Furthermore, DNA modules were developed for connecting Modular Cloning and Gateway cloning, either for toggling between systems or for standardized Gateway destination vector assembly. Finally, first instances of a "peripheral infrastructure" around Modular Cloning are presented: While available toolkits are designed for the assembly of plant transformation constructs, vectors were created to also use coding sequence-containing phytobricks directly in yeast two hybrid interaction or bacterial infection assays. The presented material will further enhance versatility of hierarchical DNA assembly strategies.
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