ArticlePloS one2018
Molecular gut content analysis of different spider body parts.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 76 citations in OpenAlex.
- Metabarcoding reveals the dietary diversity and food web structure of spider functional guilds in a highly diverse subtropical forest.Oecologia · 2026Article
- Offensive and Defensive Exploitation of Ants by Termitophagous Spiders (Araneae: Zodariidae).Integrative zoology · 2026Article
- Screening of Predatory Natural Enemies ofInsects · 2025Article
- Arthropod Food Webs in the Foreland of a Retreating Greenland Glacier: Integrating Molecular Gut Content Analysis With Structural Equation Modelling.Ecology and evolution · 2024Article
- Shift in diet composition of a riparian predator along a stream pollution gradient.Proceedings. Biological sciences · 2024Article
- What's on the menu? A novel molecular gut content analysis to investigate the feeding behavior of phytophagous insects.Ecology and evolution · 2024Article
- Dynamics of species-rich predator-prey networks and seasonal alternations of core species.Nature ecology & evolution · 2023Article
- The predator problem and PCR primers in molecular dietary analysis: Swamped or silenced; depth or breadth?Molecular ecology resources · 2023Review
- A novel molecular diagnostic method for the gut content analysis of Philaenus DNA.Scientific reports · 2022Article
- A roadmap towards predicting species interaction networks (across space and time).Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- A comparative analysis of spider prey spectra analyzed through the next-generation sequencing of individual and mixed DNA samples.Ecology and evolution · 2021Article
- Deciphering the diet of a wandering spider (Ecology and evolution · 2021Article
- Interspecific variation and functional traits of the gut microbiome in spiders from the wild: The largest effort so far.PloS one · 2021Article
- Brown spider venom toxins: what are the functions of astacins, serine proteases, hyaluronidases, allergens, TCTP, serpins and knottins?The journal of venomous animals and toxins including tropical diseases · 2021Article
- DNA metabarcoding unveils niche overlapping and competition among Caribbean sea urchins.Regional studies in marine science · 2020Article
- High-resolution multi-marker DNA metabarcoding reveals sexual dietary differentiation in a bird with minor dimorphism.Ecology and evolution · 2020Article
- Compared with conventional PCR assay, qPCR assay greatly improves the detection efficiency of predation.Ecology and evolution · 2020Article
- High-throughput sequencing for community analysis: the promise of DNA barcoding to uncover diversity, relatedness, abundances and interactions in spider communities.Development genes and evolution · 2020Review
- From metabarcoding to metaphylogeography: separating the wheat from the chaff.Ecological applications : a publication of the Ecological Society of America · 2020Article
- Beyond polyphagy and opportunism: natural prey of hunting spiders in the canopy of apple trees.PeerJ · 2020Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular gut-content analysis has revolutionized the study of food webs and feeding interactions, allowing the detection of prey DNA within the gut of many organisms. However, successful prey detection is a challenging procedure in which many factors affect every step, starting from the DNA extraction process. Spiders are liquid feeders with branched gut diverticula extending into their legs and throughout the prosoma, thus digestion takes places in different parts of the body and simple gut dissection is not possible. In this study, we investigated differences in prey detectability in DNA extracts from different parts of the spider´s body: legs, prosoma and opisthosoma, using prey-specific PCR and metabarcoding approaches. We performed feeding trials with the woodlouse hunter spider Dysdera verneaui Simon, 1883 (Dysderidae) to estimate the time at which prey DNA is detectable within the predator after feeding. Although we found that all parts of the spider body are suitable for gut-content analysis when using prey-specific PCR approach, results based on metabarcoding suggested the opisthosoma is optimal for detection of predation in spiders because it contained the highest concentration of prey DNA for longer post feeding periods. Other spiders may show different results compared to D. verneaui, but given similarities in the physiology and digestion in different families, it is reasonable to assume this to be common across species and this approach having broad utility across spiders.
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