ArticleTissue engineering. Part A2018
A Fluidic Culture Platform for Spatially Patterned Cell Growth, Differentiation, and Cocultures.
Article in Tissue engineering. Part A, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- On-Chip AC Electrothermal Pump for Pulsatile Perfusion.Micromachines · 2026Article
- Simple-Flow: A 3D-Printed Multiwell Flow Plate to Coculture Primary Human Lung Cells at the Air-Liquid Interface.ACS biomaterials science & engineering · 2025Article
- Mesenchymal Stem Cell Culture within Perfusion Bioreactors Incorporating 3D-Printed Scaffolds Enables Improved Extracellular Vesicle Yield with Preserved Bioactivity.Advanced healthcare materials · 2023Article
- Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependent.Bioengineering & translational medicine · 2023Article
- Polymeric and biological membranes for organ-on-a-chip devices.Microsystems & nanoengineering · 2023Review
- Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing.Analytica chimica acta · 2022Review
- Implementing organ-on-chip in a next-generation risk assessment of chemicals: a review.Archives of toxicology · 2022Review
- 3D Cell Cultures: Evolution of an Ancient Tool for New Applications.Frontiers in physiology · 2022Review
- Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS.Aging cell · 2021Article
- Perfused Platforms to Mimic Bone Microenvironment at the Macro/Milli/Microscale: Pros and Cons.Frontiers in cell and developmental biology · 2021Review
- Aminated 3D Printed Polystyrene Maintains Stem Cell Proliferation and Osteogenic Differentiation.Tissue engineering. Part C, Methods · 2020Article
- 3D-printed Bioreactors forInternational journal of bioprinting · 2020Review
- Dual-chambered membrane bioreactor for coculture of stratified cell populations.Biotechnology and bioengineering · 2019Article
- Assessing SSRIs' effects on fetal cardiomyocytes utilizing placenta-fetus model.Acta biomaterialia · 2019Article
- Development of surface functionalization strategies for 3D-printed polystyrene constructs.Journal of biomedical materials research. Part B, Applied biomaterials · 2019Article
- Enhanced extracellular vesicle production and ethanol-mediated vascularization bioactivity via a 3D-printed scaffold-perfusion bioreactor system.Acta biomaterialia · 2019Article
- 3D Printed Lab-on-a-Chip Platform for Chemical Stimulation and Parallel Analysis of Ion Channel Function.Micromachines · 2019Article
- New generation of bioreactors that advance extracellular matrix modelling and tissue engineering.Biotechnology letters · 2019Review
- 3D printing in cell culture systems and medical applications.Applied physics reviews · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Stem cell cultures within perfusion bioreactors, while efficient in obtaining cell numbers, often lack the similarity to native tissues and consequently cell phenotype. We develop a three-dimensional (3D)-printed fluidic chamber for dynamic stem cell culture, with emphasis on control over flow and substrate curvature in a 3D environment, two physiologic features of native tissues. The chamber geometry, consisting of an array of vertical cylindrical pillars, facilitates actin-mediated localization of human mesenchymal stem cells (hMSCs) within ∼200 μm distance from the pillars, enabling spatial patterning of hMSCs and endothelial cells in cocultures and subsequent modulation of calcium signaling between these two essential cell types in the bone marrow microenvironment. Flow-enhanced osteogenic differentiation of hMSCs in growth media imposes spatial variations of alkaline phosphatase expression, which positively correlates with local shear stress. Proliferation of hMSCs is maintained within the chamber, exceeding the cell expansion in conventional static culture. The capability to manipulate cell spatial patterning, differentiation, and 3D tissue formation through geometry and flow demonstrates the culture chamber's relevant chemomechanical cues in stem cell microenvironments, thus providing an easy-to-implement tool to study interactions among substrate curvature, shear stress, and intracellular actin machinery in the tissue-engineered construct.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.