ArticleCell journal2018
Evaluation of Hydro-Alcoholic Extract of Trifolium Pratens L. for Its Anti-Cancer Potential on U87MG Cell Line.
Article in Cell journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Trifolium pratense-Derived Exosome Improved Serum Biochemical Parameters and Pancreatic Genes in STZ-Induced Diabetic Rats.Endocrinology, diabetes & metabolism · 2025Article
- Differential Biological Effects ofAntioxidants (Basel, Switzerland) · 2024Article
- Human sperm parameter improvement associated with Ceratonia siliqua extract as a cryopreservation supplement after vitrification.Clinical and experimental reproductive medicine · 2023Article
- An In Vitro and In Vivo Assessment of Antitumor Activity of Extracts Derived from Three Well-Known Plant Species.Plants (Basel, Switzerland) · 2023Article
- Nrf2 antioxidant pathway and apoptosis induction and inhibition of NF-κB-mediated inflammatory response in human prostate cancer PC3 cells by Brassica oleracea var. acephala: An in vitro study.Molecular biology reports · 2022Article
- Improved hormonal and oxidative changes by Royal Jelly in the rat model of PCOS: An experimental study.International journal of reproductive biomedicine · 2021Article
- Relationship between Serum Levels of Anti-Mullerian Hormone, Adiponectin and Oxidative Stress Markers in Patients with Polycystic Ovary Syndrome.International journal of fertility & sterility · 2020Article
- Molecular mechanism of the anti-diabetic activity of an identified oligosaccharide fromResearch in pharmaceutical sciences · 2020Article
- Anti-proliferative and cytotoxic effect of Iranian snake (Research in pharmaceutical sciences · 2020Article
- Temozolomide and tranilast synergistic antiproliferative effect on human glioblastoma multiforme cell line (U87MG).Medical journal of the Islamic Republic of Iran · 2019Article
- Protective effect of hydroalcoholic extracts ofResearch in pharmaceutical sciences · 2018Article
- Antidiabetic effects of Peganum harmala seed extract on streptozotocin-induced diabetes in rats.Avicenna journal of phytomedicineArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveGlioblastoma multiforme is the most malignant form of brain tumors. Trifolium pratense L. has been suggested for cancer treatment in traditional medicine. Here we have investigated the effects of T. pratense extract on glioblastoma multiforme cell line (U87MG). MATERIALS AND
methodsIn this experimental study, the effect of T. pratense extract on cell viability was investigated using trypan blue staining, MTT assay, and lactate dehydrogenase activity measurement. Apoptosis and autophagy cell death were detected by fluorescent staining. Nitric oxide (No) production was measured using Griess reaction. Expression levels of some apoptotic and autophagic-related genes were detected using real-time polymerase chain reaction (PCR). The combination effects of T. pratense extract and temozolomide (TMZ) were evaluated by calculating the combination index and dose reduction index values.
resultsAfter treatment with T. pratense extract, the cell viability was significantly reduced in a time- and dosedependent manner (P<0.05). Apoptosis and autophagy of U87MG cells were significantly increased (P<0.05). Also, T. pratense extract significantly decreased NO production (P<0.05) by U87MG cells. Combination of TMZ and T. pratense extract had a synergistic cytotoxic effect.
conclusionT. pratense showed anti-cancer properties via induction of apoptosis and autophagy cell death.
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