Evidence map›Paper›PMID 29845511›Full record

ArticleJournal of molecular neuroscience : MN2018

Umbilical Cord Mesenchymal Stem Cells Conditioned Medium Promotes Aβ25-35 phagocytosis by Modulating Autophagy and Aβ-Degrading Enzymes in BV2 Cells.

Zhihao Xu, Wenbin Nan, Xiaoyue Zhang, Yuliang Sun, Jichao Yang, Kecheng Lu, Yalin Liu, Yaoxin Gao, Fen Yang, Wenchao Mao and 7 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular neuroscience : MN, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Zhihao XuStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China. zhihao220@163.com.ORCID http://orcid.org/0000-0002-0284-6742
Wenbin NanStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Xiaoyue ZhangStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Yuliang SunStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Jichao YangStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Kecheng LuStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Yalin LiuStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Yaoxin GaoStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Fen YangStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Wenchao MaoHualong Biological Technology Co. Ltd., Xinxiang, China.
Xuekun XingStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Jiang DuCollege of Biomedical Engineering, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Han LiStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Yonghai LiStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Huigen FengStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Zhiqing YuanStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China.
Juntang LinStem Cells and Biotherapy Engineering Research Center of Henan, School of Life Science and Technology, Xinxiang Medical University, East of JinSui Road, Xinxiang City, 453003, Henan Province, China. linjtlin@126.com.

Funding

Key Project of Science and Technology Research of Henan Provincial Education Department 14B180027National Natural Science Foundation of China 81771226National Natural Science Foundation of China (CN) 131600791National Training Program of Innovation and Entrepreneurship for Undergraduates of China 201610472051Postdoctoral Research Fund in Henan Province 2013041Project of Science and Technology Department of Henan Province 162102310493Scientific Research Fund of Xinxiang Medical University 2013QN122Xinxiang City Foundation CXRC16003 and ZD17008Xinxiang Medical University Foundation 20172DCG-03
6 · The paper itself

Abstract

Mesenchymal stem cell (MSC) therapy is a promising prospect for the treatment of Alzheimer's disease (AD); however, the underlying mechanisms by which MSCs mediate positive effects are still unclear. We speculated that MSCs mediate microglial autophagy and enhance the clearance of Aβ. To test this hypothesis, we cultured BV2 microglial cells with umbilical cord mesenchymal stem cells conditioned medium (ucMSCs-CM) in the presence or absence of Aβ25-35 oligomers. We investigated BV2 cell proliferation, cell death, and Aβ25-35 phagocytosis as well as protein expression levels of LC3, Beclin-1, p62, insulin-degrading enzyme (IDE), and neprilysin (Nep) with western blotting. The results showed that ucMSCs-CM inhibited the proliferation and decreased cell death of BV2 cells induced by Aβ25-35. ucMSCs-CM also promoted the phagocytosis of Aβ25-35 by BV2 cells and changed the expression of autophagy-related proteins LC3, Beclin-1, and p62. Treatment also upregulated the expression of Aβ-degrading enzymes IDE and Nep. Furthermore, the culture medium in BV2 cells with Aβ25-35 and ucMSCs-CM prevented neuronal cell SH-SY5Y from cell death compared to control medium without ucMSCs-CM. Altogether, these data suggested that ucMSCs-CM protect microglial and neuronal cells from Aβ25-35-induced cell death and promote Aβ phagocytosis by modulating autophagy and enhancing the expression of Aβ-degrading enzymes in microglia.

Indexed as

AutophagyPhagocytosisProteolysisAmyloid beta-PeptidesAnimalsBeclin-1Cell LineCell Line, TumorCells, CulturedCulture Media, ConditionedHumansInsulysinMesenchymal Stem CellsMiceMicrogliaMicrotubule-Associated ProteinsAmyloid beta-Peptidesamyloid beta-protein (25-35)Beclin-1Culture Media, ConditionedInsulysinMap1lc3b protein, mouseMicrotubule-Associated ProteinsNeprilysinPeptide FragmentsProto-Oncogene Proteins c-mycAutophagyAβConditioned mediumMesenchymal stem cellsMicroglia

Identifiers

PMID29845511

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.