ArticleCell research2018
Tissue clearing of both hard and soft tissue organs with the PEGASOS method.
Article in Cell research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 238 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
238 citing papers in PubMed, 384 citations in OpenAlex.
- Whole-mouse immunolabeling at cellular resolution for comprehensive 3D atlases.Nature protocols · 2026Review
- A scale CUBIC-based clearing protocol preserves fluorescence in mouse rib bone and cartilage.JBMR plus · 2026Article
- Accelerated immunostaining of thick biological tissues using bidirectional electric fields with reversible deformation.Biomedical optics express · 2026Article
- Identification and characterization of POSTNJournal of orthopaedic translation · 2026Article
- A targeted vector for brain endothelial cell gene delivery and cerebrovascular malformation modelling.Nature biomedical engineering · 2026Article
- Umbrella-type stretchable electrode arrays for long-term neural recording.Nature communications · 2026Article
- Comparing and Optimizing Tissue Clearing Protocols for Cochlear Immunostaining and 3D Reconstruction by Light-Sheet Microscopy.Journal of the Association for Research in Otolaryngology : JARO · 2026Article
- Osteocyte-vascular Interactions in Bone Physiology and Pathology.Current osteoporosis reports · 2026Review
- Structured illumination microscopy for high SNR 3D imaging from millimeter-thick tissues to cellular dynamics.Innovation (Cambridge (Mass.)) · 2026Article
- Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples.iScience · 2026Article
- Sensory nerve-derived signaling coordinates oropharyngeal structural organization that supports suckling and vocalization in neonatal mice.Nature communications · 2026Article
- A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.bioRxiv : the preprint server for biology · 2026Article
- PTBP1 knockdown reprograms glioma stem cells into neuronal-like cells and suppresses tumorigenesis via the DUSP5-ERK1/2 signaling pathway.Neuro-oncology · 2026Article
- Production of human blood-generating heart-forming organoids and sample preparation for advanced imaging.Nature protocols · 2026Review
- Genomic sequence evolution underlying human neocortical interareal diversification.Genome biology · 2026Article
- Advanced tissue clearing and three-dimensional imaging approaches to visualize neural innervation in the rat knee joints.Npj imaging · 2026Article
- Targeting coupling of type H-like vessels and cell fate transition to delay soft tissue aging.Nature communications · 2026Article
- Resilience of the bone marrow vascular architecture to aging and hematological stress.Blood vessels, thrombosis & hemostasis · 2026Article
- Collagen hybridizing peptide imaging and delivery of therapeutic antibody in rheumatoid arthritis models.Nature communications · 2026Article
- Comparative analysis of clearing methods for 3D imaging of the vasculature in mineralized mouse tissues.iScience · 2026Article
178 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 7 institutions in 2 countries.
Funding
Abstract
Tissue clearing technique enables visualization of opaque organs and tissues in 3-dimensions (3-D) by turning tissue transparent. Current tissue clearing methods are restricted by limited types of tissues that can be cleared with each individual protocol, which inevitably led to the presence of blind-spots within whole body or body parts imaging. Hard tissues including bones and teeth are still the most difficult organs to be cleared. In addition, loss of endogenous fluorescence remains a major concern for solvent-based clearing methods. Here, we developed a polyethylene glycol (PEG)-associated solvent system (PEGASOS), which rendered nearly all types of tissues transparent and preserved endogenous fluorescence. Bones and teeth could be turned nearly invisible after clearing. The PEGASOS method turned the whole adult mouse body transparent and we were able to image an adult mouse head composed of bones, teeth, brain, muscles, and other tissues with no blind areas. Hard tissue transparency enabled us to reconstruct intact mandible, teeth, femur, or knee joint in 3-D. In addition, we managed to image intact mouse brain at sub-cellular resolution and to trace individual neurons and axons over a long distance. We also visualized dorsal root ganglions directly through vertebrae. Finally, we revealed the distribution pattern of neural network in 3-D within the marrow space of long bone. These results suggest that the PEGASOS method is a useful tool for general biomedical research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.