ArticleScientific reports2018
Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 74 citations in OpenAlex.
- Review
- Therapeutic priming of liver regeneration: from mechanisms toFrontiers in transplantation · 2026Review
- Scalable Matrigel-Free Suspension Culture for Generating High-Quality Human Liver Ductal Organoids.Cell proliferation · 2025Article
- Single-cell morphological tracking of cell states to identify small-molecule modulators of liver differentiation.iScience · 2025Article
- Generation of Multicellular 3D Liver Organoids From Induced Pluripotent Stem Cells as a Tool for Modelling Liver Diseases.Bio-protocol · 2024Article
- Activation of ductal progenitor-like cells from adult human pancreas requires extracellular matrix protein signaling.iScience · 2024Article
- Cryopreserved cGMP-compliant human pluripotent stem cell-derived hepatic progenitors rescue mice from acute liver failure through rapid paracrine effects on liver cells.Stem cell research & therapy · 2024Article
- Identification of myeloid-derived growth factor as a mechanically-induced, growth-promoting angiocrine signal for human hepatocytes.Nature communications · 2024Article
- Liver organoid culture methods.Cell & bioscience · 2023Review
- Single-cell metabolic profiling reveals subgroups of primary human hepatocytes with heterogeneous responses to drug challenge.Genome biology · 2023Article
- Review
- Recent advances in organoid engineering: A comprehensive review.Applied materials today · 2022Article
- Optimization of 3D-aggregated spheroid model (3D-ASM) for selecting high efficacy drugs.Scientific reports · 2022Article
- Article
- Bioinspired Sandcastle Worm-Derived Peptide-Based Hybrid Hydrogel for Promoting the Formation of Liver Spheroids.Gels (Basel, Switzerland) · 2022Article
- Development ofFrontiers in immunology · 2022Article
- Hepatobiliary Organoids and Their Applications for Studies of Liver Health and Disease: Are We There Yet?Hepatology (Baltimore, Md.) · 2021Review
- Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes.Nature communications · 2021Article
- Spheroids and organoids as humanized 3D scaffold-free engineered tissues for SARS-CoV-2 viral infection and drug screening.Artificial organs · 2021Review
- Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture.Micromachines · 2021Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite decades of investigation on the proliferation of adult human primary hepatocytes, their expansion in vitro still remains challenging. To later be able to consider hepatocytes as a cell therapy alternative or bridge to liver transplantation, dramatically impeded by a shortage in liver donors, the first step is having an almost unlimited source of these cells. The banking of transplantable hepatocytes also implies a protocol for their expansion that can be compatible with large-scale production. We show that adult human primary hepatocytes when grown in 3D organoids are easily amplified, providing a substantial source of functional hepatocytes ready for transplantation. Following their plating, differentiated human hepatocytes are amplified during a transient and reversible step as liver progenitors, and can subsequently be converted back to mature differentiated hepatocytes. The protocol we propose is not only compatible with automated and high-throughput cell culture systems, thanks to the expansion of hepatocytes in suspension, but also guarantees the generation of a high number of functional cells from the same patient sample, with a relatively easy set up.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.