Evidence map›Paper›PMID 29844106›Full record

ArticleRNA (New York, N.Y.)2018

tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer-independent manner.

Canan Kuscu, Pankaj Kumar, Manjari Kiran, Zhangli Su, Asrar Malik, Anindya Dutta

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 281 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
281citing papers in PubMed, 2 pooled it
15.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

281 citing papers in PubMed, 2 syntheses or guidelines pooled it, 417 citations in OpenAlex.

  1. Pooled it
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  5. Article
  6. Flipons, Noncoding Transcripts and Retroelements: A Universal Host Defense.International journal of molecular sciences · 2026
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221 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Canan Kuscu *Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22901, USA.
Pankaj Kumar *Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22901, USA.
Manjari KiranDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22901, USA.
Zhangli SuDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22901, USA.
Asrar MalikDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22901, USA.
Anindya DuttaDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22901, USA.
University of Virginia · US

Funding

VEGF, FAK and RAPP01CA104106 · NCI · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI PASCHAL, BRYCE, THEODORESCU, DAN · 2005 to 2015
$17.9M
Regulation of TIP60 by the ubiquitin-proteasome pathwayR01GM084465 · NIGMS · UNIVERSITY OF VIRGINIA · PI DUTTA, ANINDYA · 2008 to 2017
$2.4M
NCI NIH HHS P01 CA104106NIGMS NIH HHS R01 GM084465
6 · The paper itself

Abstract

tRNA related RNA fragments (tRFs), also known as tRNA-derived RNAs (tdRNAs), are abundant small RNAs reported to be associated with Argonaute proteins, yet their function is unclear. We show that endogenous 18 nucleotide tRFs derived from the 3' ends of tRNAs (tRF-3) post-transcriptionally repress genes in HEK293T cells in culture. tRF-3 levels increase upon parental tRNA overexpression. This represses target genes with a sequence complementary to the tRF-3 in the 3' UTR. The tRF-3-mediated repression is Dicer-independent, Argonaute-dependent, and the targets are recognized by sequence complementarity. Furthermore, tRF-3:target mRNA pairs in the RNA induced silencing complex associate with GW182 proteins, known to repress translation and promote the degradation of target mRNAs. RNA-seq demonstrates that endogenous target genes are specifically decreased upon tRF-3 induction. Therefore, Dicer-independent tRF-3s, generated upon tRNA overexpression, repress genes post-transcriptionally through an Argonaute-GW182 containing RISC via sequence matches with target mRNAs.

Indexed as

Argonaute ProteinsAutoantigensCell LineDEAD-box RNA HelicasesEukaryotic Initiation FactorsGene Expression RegulationHEK293 CellsHumansProtein Processing, Post-TranslationalRibonuclease IIIRNA-Binding ProteinsRNA InterferenceRNA, MessengerRNA, Small InterferingRNA, TransferAGO1 protein, humanAGO2 protein, humanAGO3 protein, humanArgonaute ProteinsAutoantigensDEAD-box RNA HelicasesDICER1 protein, humanDROSHA protein, humanEukaryotic Initiation FactorsRibonuclease IIIRNA-Binding ProteinsRNA, MessengerRNA, Small InterferingRNA, TransferTNRC6A protein, humanpost-transcriptional gene regulationsmall noncoding RNAtRFtRNA fragments

Identifiers

PMID29844106
PMCPMC6049499
OpenAlexW2806435205

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.