ArticleBMC genomics2018
Association genetics studies on frost tolerance in wheat (Triticum aestivum L.) reveal new highly conserved amino acid substitutions in CBF-A3, CBF-A15, VRN3 and PPD1 genes.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 41 citations in OpenAlex.
- Optimizing wheat development to a range of winter climates.Journal of experimental botany · 2026Review
- Article
- Predictive breeding and marker-assisted selection for grain quality and freezing tolerance in durum wheat.Frontiers in plant science · 2026Article
- Genetic Dissection of Frost Tolerance in Winter Durum Wheat: Three Validated KASP Markers for Marker-Assisted Selection.Plants (Basel, Switzerland) · 2025Article
- Genome-wide association mapping and candidate genes analysis of high-throughput image descriptors for wheat frost tolerance.Stress biology · 2025Article
- Effect of geographical origin, regional adaptation, genotype, and release year on winter hardiness of wheat and triticale accessions evaluated for six decades in trials.Scientific reports · 2025Article
- Identification of genetic loci and candidate genes underlying freezing tolerance in wheat seedlings.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Molecular mapping of two novel cold resistance genes in common wheat by 660K SNP array.Molecular breeding : new strategies in plant improvement · 2023Article
- Genome-Wide Association Analysis of Freezing Tolerance and Winter Hardiness in Winter Wheat of Nordic Origin.Plants (Basel, Switzerland) · 2023Article
- TheCells · 2023Review
- Physiology and Molecular Breeding in Sustaining Wheat Grain Setting and Quality under Spring Cold Stress.International journal of molecular sciences · 2022Review
- Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat.BMC plant biology · 2022Article
- Genome wide association study of frost tolerance in wheat.Scientific reports · 2022Article
- Association of the Recessive AlleleFrontiers in plant science · 2022Article
- Capturing Wheat Phenotypes at the Genome Level.Frontiers in plant science · 2022Review
- Long-Amplicon Single-Molecule Sequencing Reveals Novel, Trait-Associated Variants ofFrontiers in plant science · 2022Article
- Genetic dissection of grain architecture-related traits in a winter wheat population.BMC plant biology · 2021Article
- Linkage mapping identifies a non-synonymous mutation in FLOWERING LOCUS T (FT-B1) increasing spikelet number per spike.Scientific reports · 2021Article
- AutoCloner: automatic homologue-specific primer design for full-gene cloning in polyploids.BMC bioinformatics · 2020Article
- Comparison Between Core Set Selection Methods Using Different Illumina Marker Platforms: A Case Study of Assessment of Diversity in Wheat.Frontiers in plant science · 2020Article
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Authors and funding
15 authors at 7 institutions in 2 countries.
Funding
Abstract
backgroundUnderstanding the genetic basis of frost tolerance (FT) in wheat (Triticum aestivum L.) is essential for preventing yield losses caused by frost due to cellular damage, dehydration and reduced metabolism. FT is a complex trait regulated by a number of genes and several gene families. Availability of the wheat genomic sequence opens new opportunities for exploring candidate genes diversity for FT. Therefore, the objectives of this study were to identity SNPs and insertion-deletion (indels) in genes known to be involved in frost tolerance and to perform association genetics analysis of respective SNPs and indels on FT.
resultsHere we report on the sequence analysis of 19 candidate genes for FT in wheat assembled using the Chinese Spring IWGSC RefSeq v1.0. Out of these, the tandem duplicated C-repeat binding factors (CBF), i.e. CBF-A3, CBF-A5, CBF-A10, CBF-A13, CBF-A14, CBF-A15, CBF-A18, the vernalisation response gene VRN-A1, VRN-B3, the photoperiod response genes PPD-B1 and PPD-D1 revealed association to FT in 235 wheat cultivars. Within six genes (CBF-A3, CBF-A15, VRN-A1, VRN-B3, PPD-B1 and PPD-D1) amino acid (AA) substitutions in important protein domains were identified. The amino acid substitution effect in VRN-A1 on FT was confirmed and new AA substitutions in CBF-A3, CBF-A15, VRN-B3, PPD-B1 and PPD-D1 located at highly conserved sites were detected. Since these results rely on phenotypic data obtained at five locations in 2 years, detection of significant associations of FT to AA changes in CBF-A3, CBF-A15, VRN-A1, VRN-B3, PPD-B1 and PPD-D1 may be exploited in marker assisted breeding for frost tolerance in winter wheat.
conclusionsA set of 65 primer pairs for the genes mentioned above from a previous study was BLASTed against the IWGSC RefSeq resulting in the identification of 39 primer combinations covering the full length of 19 genes. This work demonstrates the usefulness of the IWGSC RefSeq in specific primer development for highly conserved gene families in hexaploid wheat and, that a candidate gene association genetics approach based on the sequence data is an efficient tool to identify new alleles of genes important for the response to abiotic stress in wheat.
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