Evidence map›Paper›PMID 29843473›Full record

ArticleViruses2018

In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.

Nina K Broeker, Franziska Kiele, Sherwood R Casjens, Eddie B Gilcrease, Anja Thalhammer, Joachim Koetz, Stefanie Barbirz

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Structural and functional characterization of the receptor binding proteins ofComputational and structural biotechnology journal · 2021
    Article
  10. Article
  11. Phage S144, A New Polyvalent Phage InfectingInternational journal of molecular sciences · 2020
    Article
  12. Article
  13. Frontiers in microbiology · 2020
    Article
  14. Removal of Endotoxin from rAAV Samples Using a Simple Detergent-Based Protocol.Molecular therapy. Methods & clinical development · 2019
    Article
  15. Time-resolved DNA release from an O-antigen-specificThe Journal of biological chemistry · 2019
    Article
  16. Article
  17. Article
  18. Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Nina K BroekerPhysikalische Biochemie, Universität Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Golm, Germany. nina.broeker@uni-potsdam.de.
Franziska KielePhysikalische Biochemie, Universität Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Golm, Germany. frkiele@uni-potsdam.de.
Sherwood R CasjensDivision of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84112, USA. sherwood.casjens@path.utah.edu.
Eddie B GilcreaseDivision of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84112, USA. eddie.gilcrease@path.utah.edu.
Anja ThalhammerPhysikalische Biochemie, Universität Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Golm, Germany. thalhamm@uni-potsdam.de.
Joachim KoetzKolloidchemie, Universität Potsdam, Karl-Liebknecht-Str.24-25, Haus 25, 14476 Golm, Germany. koetz@uni-potsdam.de.
Stefanie BarbirzPhysikalische Biochemie, Universität Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Golm, Germany. barbirz@uni-potsdam.de.
University of Potsdam · DEUniversity of Utah · US

Funding

DNA packaging and delivery by dsDNA virusesR01GM114817 · NIGMS · UNIVERSITY OF UTAH · PI CASJENS, SHERWOOD R. · 2014 to 2017
$1.5M
NIGMS NIH HHS R01 GM114817
6 · The paper itself

Abstract

Gram-negative bacteria protect themselves with an outermost layer containing lipopolysaccharide (LPS). O-antigen-specific bacteriophages use tailspike proteins (TSP) to recognize and cleave the O-polysaccharide part of LPS. However, O-antigen composition and structure can be highly variable depending on the environmental conditions. It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance. In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the

Indexed as

Bacteriophage P22DNA, ViralEscherichia coliGlycoside HydrolasesLipopolysaccharidesPodoviridaeTemperatureViral Tail ProteinsDNA, ViralGlycoside HydrolasesLipopolysaccharidestailspike protein, bacteriophageViral Tail ProteinsHK620in vitro particle openinglipopolysaccharideO-antigen specific phagepodovirustailspike protein

Identifiers

PMID29843473
PMCPMC6024685
OpenAlexW2806944255

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.