Evidence map›Paper›PMID 29842928›Full record

ArticlePapillomavirus research (Amsterdam, Netherlands)2018

HPV E6 oncoproteins and nucleic acids in neck lymph node fine needle aspirates and oral samples from patients with oropharyngeal squamous cell carcinoma.

M Chernesky, D Jang, J Schweizer, M Arias, L Doerwald-Munoz, M Gupta, B Jackson, S Archibald, J Young, A Lytwyn and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Papillomavirus research (Amsterdam, Netherlands), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 3 pooled it
2.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 3 syntheses or guidelines pooled it, 27 citations in OpenAlex.

  1. Pooled it
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 2 countries.

M CherneskySt. Joseph's Healthcare Hamilton/ McMaster University, 50 Charlton Avenue East, Hamilton, ON, Canada L8N 4A6. Electronic address: chernesk@mcmaster.ca.
D JangSt. Joseph's Healthcare Hamilton/ McMaster University, 50 Charlton Avenue East, Hamilton, ON, Canada L8N 4A6.
J SchweizerArbor Vita Corporation, 48371 Fremont Blvd #101, Fremont, CA 94538, USA.
M AriasSt. Joseph's Healthcare Hamilton/ McMaster University, 50 Charlton Avenue East, Hamilton, ON, Canada L8N 4A6.
L Doerwald-MunozJuravinski Hospital Head & Neck Clinic, 699 Concession St, Hamilton, ON, Canada L8V 5C2.
M GuptaJuravinski Hospital Head & Neck Clinic, 699 Concession St, Hamilton, ON, Canada L8V 5C2.
B JacksonJuravinski Hospital Head & Neck Clinic, 699 Concession St, Hamilton, ON, Canada L8V 5C2.
S ArchibaldJuravinski Hospital Head & Neck Clinic, 699 Concession St, Hamilton, ON, Canada L8V 5C2.
J YoungJuravinski Hospital Head & Neck Clinic, 699 Concession St, Hamilton, ON, Canada L8V 5C2.
A LytwynSt. Joseph's Healthcare Hamilton/ McMaster University, 50 Charlton Avenue East, Hamilton, ON, Canada L8N 4A6.
M SmiejaSt. Joseph's Healthcare Hamilton/ McMaster University, 50 Charlton Avenue East, Hamilton, ON, Canada L8N 4A6.
A SeveriniNational Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, Canada R3E 3R2.
A Ecobichon-MorrisSt. Joseph's Healthcare Hamilton/ McMaster University, 50 Charlton Avenue East, Hamilton, ON, Canada L8N 4A6.
M HyrczaSt. Joseph's Healthcare Hamilton/ McMaster University, 50 Charlton Avenue East, Hamilton, ON, Canada L8N 4A6.
St. Joseph’s Healthcare Hamilton · CAJuravinski Hospital · CAArbor Vita (United States) · USPublic Health Agency of Canada · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Commercial assays measuring HPV E6 viral oncoproteins, E6/E7 mRNA or DNA were used to test neck lymph node fine needle aspirates (FNA) and oropharyngeal samples (saliva and oral swabs) from 59 Canadian patients with oropharyngeal squamous cell carcinomas (OPSCC). Overall agreements of p16 antigen staining of tumors to FNA tested for OncoE6™, Aptima HPV E6/E7 mRNA and cobas HPV DNA were 81.4% (k 0.53), 94.9% (k 0.83) and 91.1% (k 0.73) respectively. Using HPV presence in a subset of 25 tumors as the comparator, overall agreement was 64.0% (k 0.08) with OncoE6™, 88.0% (k 0.65) with Aptima HPV E6/E7 mRNA and 91.7% (k 0.70) with cobas HPV DNA. HPV testing of oropharyngeal samples yielded lower agreements with tumor markers; 23.7-24.0% (k 0.02), 55.9-68.0% (k 0.24-0.37) and 78.9-86.9% (k 0.49-0.58) in the 3 respective tests. HPV 16 was present in 93.7-100% of the samples tested and showed 100% genotype agreement between FNA and tumors. The high rates for HPV E6 oncoproteins and E6/E7 mRNA suggests most patients were experiencing transcriptionally active HPV-related OPSCC. Results from these commercial assays performed on FNA but not oropharyngeal samples showed moderate to very good agreements with p16 and HPV testing of tumors.

Indexed as

Biopsy, Fine-NeedleAdultAgedBiomarkers, TumorCanadaCarcinoma, Squamous CellFemaleHumansLymph NodesMaleMiddle AgedOncogene ProteinsOropharyngeal NeoplasmsBiomarkers, TumorOncogene Proteins

Identifiers

PMID29842928
PMCPMC5986165
OpenAlexW2803131253

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.