Evidence map›Paper›PMID 29812894›Full record

ArticleACS chemical biology2018

HaloTag Assay Suggests Common Mechanism of E. coli Membrane Permeabilization Induced by Cationic Peptides.

Zhilin Yang, James C Weisshaar

Abstract read
In one paragraph

Article in ACS chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 20 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Targeting Iron - Respiratory Reciprocity Promotes Bacterial Death.bioRxiv : the preprint server for biology · 2024
    Article
  7. Real-Time Fluorescence Microscopy on LivingInternational journal of molecular sciences · 2022
    Article
  8. Recent biomedical advances enabled by HaloTag technology.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2022
    Article
  9. Review
  10. Article
  11. Review
  12. Review
  13. Article
  14. Review
  15. Rigidification of theProceedings of the National Academy of Sciences of the United States of America · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Zhilin YangDepartment of Chemistry and Molecular Biophysics Program University of Wisconsin-Madison , 1101 University Avenue , Madison , Wisconsin 53706 , United States.
James C WeisshaarDepartment of Chemistry and Molecular Biophysics Program University of Wisconsin-Madison , 1101 University Avenue , Madison , Wisconsin 53706 , United States.ORCID 0000-0002-0922-5113
University of Wisconsin–Madison · US

Funding

Dynamics of Antimicrobial Peptide Interactions with Bacterial MembranesR01GM094510 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI WEISSHAAR, JAMES C. · 2010 to 2018
$2.2M
Design and Analysis of Random Copolymers with Antimicrobial ActivityR01GM093265 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI WEISSHAAR, JAMES C. · 2011 to 2018
$2.1M
NIGMS NIH HHS R01 GM093265NIGMS NIH HHS R01 GM094510
6 · The paper itself

Abstract

Permeabilization of the Gram-negative bacterial outer membrane (OM) by antimicrobial peptides (AMPs) is the initial step enabling access of the AMP to the cytoplasmic membrane. We present a new single-cell, time-resolved fluorescence microscopy assay that reports on the permeabilization of the E. coli OM to small molecules with a time resolution of 3 s or better. When profluorophore JF

Indexed as

Antimicrobial Cationic PeptidesCell Membrane PermeabilityEscherichia coliEscherichia coli InfectionsFluorescenceHumansMicroscopy, FluorescencePolymersSingle-Cell AnalysisAntimicrobial Cationic PeptidesPolymers

Identifiers

PMID29812894
PMCPMC6097915
OpenAlexW2806392435

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.