ArticleNature nanotechnology2018
DNA scaffolds support stable and uniform peptide nanopores.
Article in Nature nanotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Large-Diameter DNA-Scaffolded Nanopores Enabled by Loosely Packed Peptides for Single-Molecule Sensing.Angewandte Chemie (International ed. in English) · 2026Article
- Membrane-Spanning Nanopores Formed from Nucleic Acids.Chemical reviews · 2026Review
- Article
- A non-B DNA binding peptidomimetic channel alters cellular functions.Nature communications · 2024Article
- Deconvoluting the Effect of Cell-Penetrating Peptides for Enhanced and Controlled Insertion of Large-Scale DNA Nanopores.ACS applied materials & interfaces · 2024Article
- Lipid vesicle-based molecular robots.Lab on a chip · 2024Review
- Molecular Recognition in Confined Space Elucidated with DNA Nanopores and Single-Molecule Force Microscopy.Nano letters · 2023Article
- Recent Advances in DNA Origami-Engineered Nanomaterials and Applications.Chemical reviews · 2023Review
- Light-Activated Assembly of Connexon Nanopores in Synthetic Cells.Journal of the American Chemical Society · 2023Article
- Functionalized DNA-Origami-Protein Nanopores Generate Large Transmembrane Channels with Programmable Size-Selectivity.Journal of the American Chemical Society · 2023Article
- A Light-Triggered Synthetic Nanopore for Controlling Molecular Transport Across Biological Membranes.Angewandte Chemie (International ed. in English) · 2022Article
- Synergistic DNA- and Protein-Based Recognition Promote an RNA-Templated Bio-orthogonal Reaction.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Constructing ion channels from water-soluble α-helical barrels.Nature chemistry · 2021Article
- Structure-flexible DNA origami translocation through a solid-state nanopore.RSC advances · 2021Article
- Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics.APL bioengineering · 2020Review
- Designed alpha-helical barrels for charge-selective peptide translocation.Chemical science · 2020Article
- Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports KNature communications · 2020Article
- Engineering Lipid Membranes with Programmable DNA Nanostructures.Advanced biosystems · 2020Review
- The Fusion of Lipid and DNA Nanotechnology.Genes · 2019Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of peptides into membrane-spanning nanopores might be promoted by scaffolds to pre-organize the structures. Such scaffolds could enable the construction of uniform pores of various sizes and pores with controlled permutations around a central axis. Here, we show that DNA nanostructures can serve as scaffolds to arrange peptides derived from the octameric polysaccharide transporter Wza to form uniform nanopores in planar lipid bilayers. Our ring-shaped DNA scaffold is assembled from short synthetic oligonucleotides that are connected to Wza peptides through flexible linkers. When scaffolded, the Wza peptides form conducting nanopores of which only octamers are stable and of uniform conductance. Removal of the DNA scaffold by cleavage of the linkers leads to a rapid loss of the nanopores from the lipid bilayer, which shows that the scaffold is essential for their stability. The DNA scaffold also adds functionality to the nanopores by enabling reversible and permanent binding of complementary tagged oligonucleotides near the nanopore entrance.
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Registered trials
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