Evidence map›Paper›PMID 29807908›Full record

ArticleMolecular systems biology2018

Mapping DNA damage-dependent genetic interactions in yeast via party mating and barcode fusion genetics.

J Javier Díaz-Mejía, Albi Celaj, Joseph C Mellor, Atina Coté, Attila Balint, Brandon Ho, Pritpal Bansal, Fatemeh Shaeri, Marinella Gebbia, Jochen Weile and 11 more

Open access · goldAbstract read
In one paragraph

Article in Molecular systems biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 26 citations in OpenAlex.

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  18. Two protein/protein interaction assays in one go.Molecular systems biology · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 4 institutions in 2 countries.

J Javier Díaz-MejíaDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Albi CelajDonnelly Centre, University of Toronto, Toronto, ON, Canada.ORCID 0000-0002-5888-772X
Joseph C MellorDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Atina CotéDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Attila BalintDonnelly Centre, University of Toronto, Toronto, ON, Canada.ORCID 0000-0002-6481-1772
Brandon HoDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Pritpal BansalDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Fatemeh ShaeriDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Marinella GebbiaDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Jochen WeileDonnelly Centre, University of Toronto, Toronto, ON, Canada.ORCID 0000-0003-1628-9390
Marta VerbyDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Anna KarkhaninaDonnelly Centre, University of Toronto, Toronto, ON, Canada.
YiFan ZhangDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Cassandra WongLunenfeld-Tanenbaum Research Institute, Mt. Sinai Hospital, Toronto, ON, Canada.
Justin RichDonnelly Centre, University of Toronto, Toronto, ON, Canada.
D'Arcy PrendergastDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Gaurav GuptaDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Sedide ÖztürkDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Daniel DurocherDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Grant W BrownDonnelly Centre, University of Toronto, Toronto, ON, Canada.
Frederick P RothDonnelly Centre, University of Toronto, Toronto, ON, Canada fritz.roth@utoronto.ca.ORCID 0000-0002-6628-649X
University of Toronto · CAHarvard University · USCanadian Institute for Advanced Research · CALunenfeld-Tanenbaum Research Institute · CA

Funding

Genomic Analysis of Network Perturbations in Human DiseaseP50HG004233 · NHGRI · DANA-FARBER CANCER INST · PI VIDAL, MARC · 2007 to 2017
$31.6M
The C. elegans Interactome ProjectR01HG001715 · NHGRI · MASSACHUSETTS GENERAL HOSPITAL · PI HILL, DAVID E., ROTH, FREDERICK P · 1998 to 2010
$16.5M
Mapping the first half of the REFERENCE human binary protein interactomeU01HG001715 · NHGRI · DANA-FARBER CANCER INST · PI HILL, DAVID E., ROTH, FREDERICK P · 2012 to 2014
$5.4M
Resource ProjectU41HG001715 · NHGRI · DANA-FARBER CANCER INST · PI HILL, DAVID E. · 2015 to 2017
$4.7M
Technology for large-scale genetic interaction discovery in S. cerevisiaeR21HG004756 · NHGRI · HARVARD MEDICAL SCHOOL · PI ROTH, FREDERICK P · 2008 to 2009
$466k
Interrogating Genetic Interaction Networks via Next-Generation SequencingF32HG004825 · NHGRI · HARVARD MEDICAL SCHOOL · PI MELLOR, JOSEPH C · 2008 to 2010
$97k
CIHR FDN143343CIHR MOP-79368NHGRI NIH HHS F32 HG004825NHGRI NIH HHS P50 HG004233NHGRI NIH HHS R01 HG001715NHGRI NIH HHS R21 HG004756NHGRI NIH HHS U01 HG001715NHGRI NIH HHS U41 HG001715
6 · The paper itself

Abstract

Condition-dependent genetic interactions can reveal functional relationships between genes that are not evident under standard culture conditions. State-of-the-art yeast genetic interaction mapping, which relies on robotic manipulation of arrays of double-mutant strains, does not scale readily to multi-condition studies. Here, we describe barcode fusion genetics to map genetic interactions (BFG-GI), by which double-mutant strains generated via

Indexed as

Chromosome MappingDNA Barcoding, TaxonomicDNA DamageDNA RepairEpistasis, GeneticGene DeletionGenetic LociHigh-Throughput Nucleotide SequencingMethyl MethanesulfonateModels, TheoreticalPromoter Regions, GeneticReproducibility of ResultsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsMethyl MethanesulfonateSaccharomyces cerevisiae Proteinscondition‐dependentDNA barcodeen massegenetic interactionsequencing

Identifiers

PMID29807908
PMCPMC5974512
OpenAlexW2951693812

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.