ArticleMetabolic engineering2018
Anaerobic production of medium-chain fatty alcohols via a β-reduction pathway.
Article in Metabolic engineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Engineering New-to-Nature Biological Pathways for β,γ-Alkanediol Synthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Engineering Escherichia coli to produce medium chain oleochemicals from C2 substrates.Journal of industrial microbiology & biotechnology · 2026Article
- Demonstration and technoeconomic analysis of dodecanol production from acetate using metabolically engineered Escherichia coli.Metabolic engineering · 2026Article
- A metabolic engineering strategy for producing poly-(3-hydroxyoctanoic acid) in Escherichia coli from glycerol.Metabolic engineering · 2025Article
- Multiscale regulation of nutrient stress responses in Escherichia coli from chromatin structure to small regulatory RNAs.Nucleic acids research · 2025Article
- Decoding the role of the arginine dihydrolase pathway in shaping human gut community assembly and health-relevant metabolites.Cell systems · 2025Article
- Concentration-Dependent CsrA Regulation of theACS synthetic biology · 2025Article
- Genomic Insights into the Role of cAMP in Carotenoid Biosynthesis: Enhancing β-Carotene Production inInternational journal of molecular sciences · 2024Article
- Genome reduction improves octanoic acid production in scale down bioreactors.Microbial biotechnology · 2024Article
- Manipulating the molecular specificity of transcriptional biosensors for tryptophan metabolites and analogs.Cell reports. Physical science · 2024Article
- Rewiring native post-transcriptional global regulators to achieve designer, multi-layered genetic circuits.Nature communications · 2024Article
- Biosynthesis of C4-C8 3-Hydroxycarboxylic Acids from Glucose through the Inverted Fatty Acid β-Oxidation by Metabolically EngineeredBiomolecules · 2024Article
- The Post-Transcriptional Regulatory Protein CsrA Amplifies Its Targetome through Direct Interactions with Stress-Response Regulatory Hubs: The EvgA and AcnA Cases.Microorganisms · 2024Article
- Overcoming barriers to medium-chain fatty alcohol production.Current opinion in biotechnology · 2024Review
- Evaluating the feasibility of medium-chain oleochemical synthesis using microbial chain elongation.Journal of industrial microbiology & biotechnology · 2024Article
- Complete microbial synthesis of crocetin and crocins from glycerol in Escherichia coli.Microbial cell factories · 2024Article
- Engineering E. coli strains using antibiotic-resistance-gene-free plasmids.Cell reports methods · 2023Article
- Relative Activities of the β-ketoacyl-CoA and Acyl-CoA Reductases Influence Product Profile and Flux in a Reversed β-Oxidation Pathway.ACS catalysis · 2023Article
- Characterizing and utilizing oxygen-dependent promoters for efficient dynamic metabolic engineering.Metabolic engineering · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In this report, we identify the relevant factors to increase production of medium chain n-alcohols through an expanded view of the reverse β-oxidation pathway. We began by creating a base strain capable of producing medium chain n-alcohols from glucose using a redox-balanced and growth-coupled metabolic engineering strategy. By dividing the heterologous enzymes in the pathway into different modules, we were able to identify and evaluate homologs of each enzyme within the pathway and identify several capable of enhancing medium chain alcohol titers and/or selectivity. In general, the identity of the trans-2-enoyl-CoA reductase (TER) and the direct overexpression of the thiolase (FadA) and β-hydroxy-acyl-CoA reductase (FadB) improved alcohol titer and the identity of the FadBA complex influenced the dominant chain length. Next, we linked the anaerobically induced VHb promoter from Vitreoscilla hemoglobin to each gene to remove the need for chemical inducers and ensure robust expression. The highest performing strain with the autoinduced reverse β-oxidation pathway produced n-alcohols at titers of 1.8 g/L with an apparent molar yield of 0.2 on glucose consumed in rich medium (52% of theoretical yield).
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Registered trials
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