ArticleParasitology2018
Whole genome amplification and exome sequencing of archived schistosome miracidia.
Article in Parasitology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- Resistance evaluation and surveillance initiative for schistosomiasis treatment: study protocol for the RESIST project.BMC infectious diseases · 2026Article
- Efficient amplicon panels for evaluating hybridization and genetic structure in the Schistosoma haematobium species group.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2026Article
- Pooled, Long-read Sequencing for Structural Variant Characterization in Schistosome Populations.Genome biology and evolution · 2025Article
- The research contribution of the Schistosomiasis Collection at the Natural History Museum (SCAN): highlights, challenges and future directions.Infectious diseases of poverty · 2025Review
- Genomic data reveal a north-south split and introgression history of blood fluke populations across Africa.Nature communications · 2025Article
- How useful is Nanopore adaptive sampling for sequencingWellcome open research · 2025Article
- Hybrid-Capture Target Enrichment in Human Pathogens: Identification, Evolution, Biosurveillance, and Genomic Epidemiology.Pathogens (Basel, Switzerland) · 2024Review
- Integrating genomic and epidemiologic data to accelerate progress toward schistosomiasis elimination.eLife · 2022Review
- Genome-wide analysis of Schistosoma mansoni reveals limited population structure and possible praziquantel drug selection pressure within Ugandan hot-spot communities.PLoS neglected tropical diseases · 2022Article
- Genomic analysis of a parasite invasion: Colonization of the Americas by the blood fluke Schistosoma mansoni.Molecular ecology · 2022Article
- Genetic analysis of praziquantel response in schistosome parasites implicates a transient receptor potential channel.Science translational medicine · 2021Article
- Genetic architecture of transmission stage production and virulence in schistosome parasites.Virulence · 2021Article
- Patterns of relatedness and genetic diversity inferred from whole genome sequencing of archival blood fluke miracidia (Schistosoma japonicum).PLoS neglected tropical diseases · 2021Article
- Snail-Related Contributions from the Schistosomiasis Consortium for Operational Research and Evaluation Program Including Xenomonitoring, Focal Mollusciciding, Biological Control, and Modeling.The American journal of tropical medicine and hygiene · 2020Review
- Parasite Population Genetic Contributions to the Schistosomiasis Consortium for Operational Research and Evaluation within Sub-Saharan Africa.The American journal of tropical medicine and hygiene · 2020Review
- Oxamniquine resistance alleles are widespread in Old World Schistosoma mansoni and predate drug deployment.PLoS pathogens · 2019Article
- Ancient Hybridization and Adaptive Introgression of an Invadolysin Gene in Schistosome Parasites.Molecular biology and evolution · 2019Article
- Evaluation of DNA Extraction Methods on Individual Helminth Egg and Larval Stages for Whole-Genome Sequencing.Frontiers in genetics · 2019Article
- Whole-genome sequence of the bovine blood fluke Schistosoma bovis supports interspecific hybridization with S. haematobium.PLoS pathogens · 2019Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
Adult schistosomes live in the blood vessels and cannot easily be sampled from humans, so archived miracidia larvae hatched from eggs expelled in feces or urine are commonly used for population genetic studies. Large collections of archived miracidia on FTA cards are now available through the Schistosomiasis Collection at the Natural History Museum (SCAN). Here we describe protocols for whole genome amplification of Schistosoma mansoni and Schistosome haematobium miracidia from these cards, as well as real time PCR quantification of amplified schistosome DNA. We used microgram quantities of DNA obtained for exome capture and sequencing of single miracidia, generating dense polymorphism data across the exome. These methods will facilitate the transition from population genetics, using limited numbers of markers to population genomics using genome-wide marker information, maximising the value of collections such as SCAN.
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Registered trials
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