Evidence map›Paper›PMID 29805845›Full record

ArticleNature biomedical engineering2017

Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA

Kunwoo Lee, Michael Conboy, Hyo Min Park, Fuguo Jiang, Hyun Jin Kim, Mark A Dewitt, Vanessa A Mackley, Kevin Chang, Anirudh Rao, Colin Skinner and 12 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biomedical engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 386 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
386citing papers in PubMed, 2 pooled it
31.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

386 citing papers in PubMed, 2 syntheses or guidelines pooled it, 730 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Review
  6. CRISPR/Cas9-based nanodelivery systems for precision gene editing in neuroinflammation.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026
    Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. SORT LNPs encapsulating Cas9 mRNA achieve efficient editing in skeletal muscle in a dystrophic mouse model.Molecular therapy : the journal of the American Society of Gene Therapy · 2026
    Article
  13. Review
  14. Review
  15. Article
  16. Leveraging Macrophage Metabolic Reprogramming for Enhanced Anti-Tumor Immunity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  17. Review
  18. Review
  19. A modified CRISPR/Cas9 approach in silencing the triplication in Down syndrome: A treatment path XISTs.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  20. CRISPR and the Future of Cardiac Disease Therapy: A New Genetic Frontier.International journal of molecular sciences · 2026
    Review

326 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 5 institutions in 2 countries.

Kunwoo LeeGenEdit, Berkeley, CA, 94720-0001, USA.
Michael ConboyDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Hyo Min ParkGenEdit, Berkeley, CA, 94720-0001, USA.
Fuguo JiangDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Hyun Jin KimDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Mark A DewittDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Vanessa A MackleyGenEdit, Berkeley, CA, 94720-0001, USA.
Kevin ChangDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Anirudh RaoDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Colin SkinnerDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Tamanna ShobhaDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Melod MehdipourDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Hui LiuGenEdit, Berkeley, CA, 94720-0001, USA.
Wen-Chin HuangDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Freeman LanDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Nicolas L BrayDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Song LiDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Jacob E CornDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID http://orcid.org/0000-0002-7798-5309
Kazunori KataokaCenter for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, 113-0033, Japan.
Jennifer A DoudnaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Irina ConboyDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA. iconboy@berkeley.edu.
Niren MurthyDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA. nmurthy@berkeley.edu.
University of California, Berkeley · USInnovative Genomics Institute · USHoward Hughes Medical Institute · USInnovation Center of NanoMedicine · JPThe University of Tokyo · JP

Funding

Spatiotemporal control of reactive oxygen species in T cellsR01AI088023 · NIAID · GEORGIA INSTITUTE OF TECHNOLOGY · PI KEMP, MELISSA LAMBETH · 2011 to 2014
$1.4M
Treatment of Duchenne Muscular Dystrophy with Cas9 Protein Complexed to Gold NanoparticlesR01EB023776 · NIBIB · UNIVERSITY OF CALIFORNIA BERKELEY · PI CONBOY, IRINA M, MURTHY, NIREN · 2017 to 2020
$1.4M
Rapid antimicrobial susceptibility determination of bacterial pathogensR01AI107116 · NIAID · GEORGIA INSTITUTE OF TECHNOLOGY · PI DICKSON, ROBERT M, TZENG, YIH-LING · 2014 to 2017
$1.4M
Genome Sequencer FLXS10RR029668 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI NGAI, JOHN J. · 2010 to 2010
$623k
Illumina Sequencer to Facilitate Functional Genomics at BerkeleyS10RR027303 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI TAYLOR, JOHN W. · 2010 to 2010
$500k
Rapid antimicrobial susceptibility determination of bacterial pathogensR56AI107116 · NIAID · GEORGIA INSTITUTE OF TECHNOLOGY · PI DICKSON, ROBERT M, TZENG, YIH-LING · 2013 to 2013
$379k
Identification of young versus aged proteomes by tagging with non-canonical aminoR56AG048316 · NIA · UNIVERSITY OF CALIFORNIA BERKELEY · PI CONBOY, IRINA M, TIRRELL, DAVID A · 2014 to 2014
$156k
NCRR NIH HHS S10 RR027303NCRR NIH HHS S10 RR029668NIAID NIH HHS R01 AI088023NIAID NIH HHS R01 AI107116NIAID NIH HHS R56 AI107116NIA NIH HHS R56 AG048316NIBIB NIH HHS R01 EB023776
6 · The paper itself

Abstract

CRISPR/Cas9-based therapeutics, especially those that can correct gene mutations via homology directed repair (HDR), have the potential to revolutionize the treatment of genetic diseases. However, HDR-based therapeutics are challenging to develop because they require simultaneous

Identifiers

PMID29805845
PMCPMC5968829
OpenAlexW2759402071

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.