ArticleJournal of ophthalmology2018
Generation of Femtosecond Laser-Cut Decellularized Corneal Lenticule Using Hypotonic Trypsin-EDTA Solution for Corneal Tissue Engineering.
Article in Journal of ophthalmology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Preservation and Reuse of KLEx Lenticules: From Biobanking to Therapeutic and Refractive Innovations.Bioengineering (Basel, Switzerland) · 2026Review
- Decellularized corneal-based 3D scaffolds: methods decellularization, characterization, mechanical properties, and species source.Regenerative therapy · 2026Review
- Advances in Decellularization of Fish Wastes for Extracellular Matrix Extraction in Sustainable Tissue Engineering and Regenerative Medicine.Bioengineering (Basel, Switzerland) · 2026Review
- Bioartificial human corneas generated by tissue engineering. A historical and technical review.Histology and histopathology · 2026Review
- Decellularized apple hypanthium as a plant-based biomaterial for cartilage regeneration in vitro: a comparative study of progenitor cell types and environmental conditions.Journal of biological engineering · 2025Article
- Decellularization and Their Significance for Tissue Regeneration in the Era of 3D Bioprinting.ACS omega · 2024Review
- Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.Bioactive materials · 2024Review
- Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature.Journal of ophthalmology · 2024Review
- Development potential of extracellular matrix hydrogels as hemostatic materials.Frontiers in bioengineering and biotechnology · 2023Review
- Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering.Bioactive materials · 2022Review
- Application of decellularized vascular matrix in small-diameter vascular grafts.Frontiers in bioengineering and biotechnology · 2022Review
- Bioengineered Human Stromal Lenticule for Recombinant Human Nerve Growth Factor Release: A Potential Biocompatible Ocular Drug Delivery System.Frontiers in bioengineering and biotechnology · 2022Article
- Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods.Frontiers in bioengineering and biotechnology · 2022Review
- Banking of corneal stromal lenticules: a risk-analysis assessment with the EuroGTP II interactive tool.Cell and tissue banking · 2020Article
- Decellularization methods for developing porcine corneal xenografts and future perspectives.Xenotransplantation · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo establish an optimized and standardized protocol for the development of optimal scaffold for bioengineering corneal substitutes, we used femtosecond laser to process human corneal tissue into stromal lenticules and studied to find the most efficient decellularization method among various reagents with different tonicities.
methodsThe decellularization efficacy of several agents (0.1%, 0.25%, and 0.5% of Triton X-100, SDS, and trypsin-EDTA (TE), resp.) with different tonicities was evaluated. Of all protocols, the decellularization methods, which efficiently removed nuclear materials examined as detected by immunofluorescent staining, were quantitatively tested for sample DNA and glycosaminoglycan (GAG) contents, recellularization efficacy, and biocompatibilities.
results0.5% SDS in hypertonic and isotonic buffer, 0.25% TE in hypotonic buffer, and 0.5% TE in all tonicities completely decellularized the corneal lenticules. Of the protocols, decellularization with hypotonic 0.25 and 0.5% TE showed the lowest DNA contents, while the GAG content was the highest. Furthermore, the recellularization efficacy of the hypotonic TE method was better than that of the SDS-based method. Hypotonic TE-treated decellularized corneal lenticules (DCLs) were sufficiently transparent and biocompatible.
conclusionWe generated an ideal protocol for DCLs using a novel method. Furthermore, it is possible to create a scaffold using a bioengineered corneal substitute.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.