Evidence map›Paper›PMID 29805497›Full record

ArticleExperimental and therapeutic medicine2018

Protective effects of human induced pluripotent stem cell-derived exosomes on high glucose-induced injury in human endothelial cells.

Qianqian Ding, Ruiting Sun, Pingping Wang, Heng Zhang, Meng Xiang, Dan Meng, Ning Sun, Alex F Chen, Sifeng Chen

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental and therapeutic medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 28 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. State of the field: cellular and exosomal therapeutic approaches in vascular regeneration.American journal of physiology. Heart and circulatory physiology · 2022
    Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Stem Cell Extracellular Vesicles and their Potential to Contribute to the Repair of Damaged CNS Cells.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2020
    Article
  13. Review
  14. Review
  15. Utilization of Human Induced Pluripotent Stem Cells for Cardiac Repair.Frontiers in cell and developmental biology · 2020
    Review
  16. Article
  17. ALIX increases protein content and protective function of iPSC-derived exosomes.Journal of molecular medicine (Berlin, Germany) · 2019
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Qianqian DingDepartment of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.
Ruiting SunDepartment of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.
Pingping WangDepartment of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.
Heng ZhangDepartment of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.
Meng XiangDepartment of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.
Dan MengDepartment of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.
Ning SunDepartment of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.
Alex F ChenDepartment of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.
Sifeng ChenDepartment of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China.
Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes are a family of extracellular vesicles that are secreted from almost all types of cells and are associated with cell-to-cell communication. The present study was performed to investigate the effects of human induced pluripotent stem cell-derived exosomes (hiPSC-exo) on cell viability, capillary-like structure formation and senescence in endothelial cells exposed to high glucose. Exosomes were isolated from the conditional medium of hiPSCs and confirmed by transmission electron microscopy, nanoparticle tracking analysis and western blot analysis using Alix and cluster of differentiation-63 as markers. hiPSC-exo were labeled with PKH26 for tracking, and it was determined that spherical exosomes, with a typical cup-shape, were absorbed by human umbilical vascular endothelial cells (HUVECs). Cultured HUVECs were treated with high glucose (33 mM) with or without hiPSC-exo (20 µg/ml) for 48 h, and cell viability, capillary tube formation and senescence were assessed. When exposed to high glucose, viability and tube formation in HUVECs was significantly reduced (P<0.0001), whereas the proportion of senescent cells was higher compared with that in control HUVECs (P<0.0001). Furthermore, hiPSC-exo restored cell viability and capillary-like structure formation, and reduced senescence in HUVECs exposed to high glucose (P<0.0001). However, hiPSC-exo had minimal effects on normal HUVECs. These findings suggest that stem cell-derived exosomes are able to promote cell proliferation, enhance capillary-like structure formation and reduce senescence in endothelial cells exposed to high glucose.

Indexed as

endothelial cellsexosomeshigh glucoseinduced pluripotent stem cellsrepair

Identifiers

PMID29805497
PMCPMC5958753
OpenAlexW2797032954

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.