ArticleInternational journal of biological sciences2018
The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium.
Article in International journal of biological sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Morphogen-Directed, High-Throughput Development of hiPSC-Derived Telencephalic Organoids for Comparative In Vitro Phenotyping.Current protocols · 2026Article
- EBNEO Commentary: Late Permissive Hypercapnia for Mechanically Ventilated Preterm Infants.Acta paediatrica (Oslo, Norway : 1992) · 2025Article
- Establishing an Ex Vivo Culture Model of Human Proximal Airway Tissue.Methods and protocols · 2025Article
- Remodeling Cell Adhesion Releases Cardiac Potential of Human Pluripotent Stem Cells with Continuous Proliferation and Accelerated Maturation.International journal of biological sciences · 2025Article
- Gain of 1q confers an MDM4-driven growth advantage to undifferentiated and differentiating hESC while altering their differentiation capacity.Cell death & disease · 2024Article
- Medium acidosis drives cardiac differentiation during mesendoderm cell fate specification from human pluripotent stem cells.Stem cell reports · 2024Article
- Article
- Gains of 20q11.21 in human pluripotent stem cells: Insights from cancer research.Stem cell reports · 2024Review
- Influence of acidic metabolic environment on differentiation of stem cell-derived cardiomyocytes.Frontiers in cardiovascular medicine · 2024Review
- TPX2 Amplification-Driven Aberrant Mitosis in Culture Adapted Human Embryonic Stem Cells with gain of 20q11.21.Stem cell reviews and reports · 2023Article
- Development and evaluation of a novel xeno-free culture medium for human-induced pluripotent stem cells.Stem cell research & therapy · 2022Article
- Thyroid hormone enhances stem cell maintenance and promotes lineage-specific differentiation in human embryonic stem cells.Stem cell research & therapy · 2022Article
- Insulin Directs Dichotomous Translational Regulation to Control Human Pluripotent Stem Cell Survival, Proliferation and Pluripotency.International journal of biological sciences · 2022Article
- Regulation of energy metabolism in human pluripotent stem cells.Cellular and molecular life sciences : CMLS · 2021Review
- Nicotinamide promotes cardiomyocyte derivation and survival through kinase inhibition in human pluripotent stem cells.Cell death & disease · 2021Article
- The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells.Cells · 2021Review
- Induced pluripotent stem cells as suitable sensors for fibromyalgia and myalgic encephalomyelitis/chronic fatigue syndrome.World journal of stem cells · 2021Article
- GPR4 signaling is essential for the promotion of acid-mediated angiogenic capacity of endothelial progenitor cells by activating STAT3/VEGFA pathway in patients with coronary artery disease.Stem cell research & therapy · 2021Article
- Using Live Imaging and FUCCI Embryonic Stem Cells to Rank DevTox Risks: Adverse Growth Effects of PFOA Compared With DEP Are 26 Times Faster, 1,000 Times More Sensitive, and 13 Times Greater in Magnitude.Frontiers in toxicology · 2021Article
- Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture.Stem cell reports · 2020Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell density has profound impacts on the cell culture practices of human pluripotent stem cells. The regulation of cell growth, cell death, pluripotency and differentiation converge at high density, but it is largely unknown how different regulatory mechanisms act at this stage. We use a chemically defined medium to systemically examine cellular activities and the impact of medium components in high-density culture. We show that medium acidosis is the main factor that alters cell cycle, gene expression and cellular metabolism at high cell density. The low medium pH leads to inhibition of glucose consumption, cell cycle arrest, and subsequent cell death. At high cell density, the suppression of medium acidosis with sodium bicarbonate (NaHCO
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.