ArticleMethods in cell biology2018
Employing the one-cell C. elegans embryo to study cell division processes.
Article in Methods in cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Cyclin B3-CDK1 couples translational de-repression to embryonic mitoses via OMA protein degradation inbioRxiv : the preprint server for biology · 2026Article
- An interkinetic envelope surrounds chromosomes between meiosis I and II in C. elegans oocytes.The Journal of cell biology · 2025Article
- 4D Microscopy and Tracking of Chromosomes and the Spindle in C. elegans Early Embryos.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses.The Journal of cell biology · 2024Article
- The CYK-4 GAP domain regulates cortical targeting of centralspindlin to promote contractile ring assembly and facilitate ring dissolution.bioRxiv : the preprint server for biology · 2024Article
- An interkinetic envelope surrounds chromosomes between meiosis I and II inbioRxiv : the preprint server for biology · 2024Article
- Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome.The Journal of cell biology · 2021Article
- A tripartite mechanism catalyzes Mad2-Cdc20 assembly at unattached kinetochores.Science (New York, N.Y.) · 2021Article
- A Non-canonical BRCT-Phosphopeptide Recognition Mechanism Underlies RhoA Activation in Cytokinesis.Current biology : CB · 2020Article
- The G2-to-M Transition Is Ensured by a Dual Mechanism that Protects Cyclin B from Degradation by Cdc20-Activated APC/C.Developmental cell · 2019Article
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Authors and funding
12 authors.
Funding
Abstract
The one-cell Caenorhabditis elegans embryo offers many advantages for mechanistic analysis of cell division processes. Conservation of key genes and pathways involved in cell division makes findings in C. elegans broadly relevant. A key technical advantage of this system is the ability to penetrantly deplete essential gene products by RNA interference (RNAi) and replace them with wild-type or mutant versions expressed at endogenous levels from single copy RNAi-resistant transgene insertions. This ability to precisely perturb essential genes is complemented by the inherently highly reproducible nature of the zygotic division that facilitates development of quantitative imaging assays. Here, we detail approaches to generate targeted single copy transgene insertions that are RNAi-resistant, to engineer variants of individual genes employing transgene insertions as well as at the endogenous locus, and to in situ tag genes with fluorophores/purification tags. We also describe imaging assays and common image analysis tools employed to quantitatively monitor phenotypic effects of specific perturbations on meiotic and mitotic chromosome segregation, centrosome assembly/function, and cortical dynamics/cytokinesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.