ArticleMolecular plant2018
Structural Flexibility Enables Alternative Maturation, ARGONAUTE Sorting and Activities of miR168, a Global Gene Silencing Regulator in Plants.
Article in Molecular plant, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed.
- An optimized protocol for plant extracellular vesicles isolation from Ophiopogon japonicus root: a comparative evaluation based on miRNA cargo.Plant methods · 2025Article
- Oxidative stress: a new player in the regulation of AGO1 homeostasis?Journal of experimental botany · 2025Article
- Remote precursor elements can modulate RNA induced silencing complex-loading efficiency of miR168 in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- Review
- athisomiRDB: A comprehensive database of Arabidopsis isomiRs.Database : the journal of biological databases and curation · 2024Article
- Principles of miRNA/miRNA* function in plant MIRNA processing.Nucleic acids research · 2024Article
- ARGONAUTE10 controls cell fate specification and formative cell divisions in the Arabidopsis root.The EMBO journal · 2024Article
- An AGO10:miR165/6 module regulates meristem activity and xylem development in the Arabidopsis root.The EMBO journal · 2024Article
- Beyond Loading: Functions of Plant ARGONAUTE Proteins.International journal of molecular sciences · 2023Review
- The plant noncoding transcriptome: a versatile environmental sensor.The EMBO journal · 2023Review
- Regulation of plant immunity via small RNA-mediated control of NLR expression.Journal of experimental botany · 2023Article
- The Identification and Comparative Analysis of Non-Coding RNAs in Spores and Mycelia ofJournal of fungi (Basel, Switzerland) · 2023Article
- microRNA biogenesis and stabilization in plants.Fundamental research · 2023Review
- miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensuresScience advances · 2023Article
- A contemporary reassessment of the enhanced transient expression system based on the tombusviral silencing suppressor protein P19.The Plant journal : for cell and molecular biology · 2023Article
- Plant gene silencing signals move from the phloem to influence gene expression in shoot apical meristems.BMC plant biology · 2022Article
- Suppression of both intra- and intercellular RNA silencing by the tombusviral P19 protein requires its small RNA binding property.The New phytologist · 2022Article
- RNA and Protein Determinants Mediate Differential Binding of miRNAs by a Viral Suppressor of RNA Silencing Thus Modulating Antiviral Immune Responses in Plants.International journal of molecular sciences · 2022Article
- CRISPR-Cas9 mediated OsMIR168a knockout reveals its pleiotropy in rice.Plant biotechnology journal · 2022Article
- Controlled RISC loading efficiency of miR168 defined by miRNA duplex structure adjusts ARGONAUTE1 homeostasis.Nucleic acids research · 2021Article
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8 authors.
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Abstract
In eukaryotes, the RNase-III Dicer often produces length/sequence microRNA (miRNA) variants, called "isomiRs", owing to intrinsic structural/sequence determinants of the miRNA precursors (pre-miRNAs). In this study, we combined biophysics, genetics and biochemistry approaches to study Arabidopsis miR168, the key feedback regulator of central plant silencing effector protein ARGONAUTE1 (AGO1). We identified a motif conserved among plant pre-miR168 orthologs, which enables flexible internal base-pairing underlying at least three metastable structural configurations. These configurations promote alternative, accurate Dicer cleavage events generating length and structural isomiR168 variants with distinctive AGO sorting properties and modes of action. Among these isomiR168s, a duplex with a 22-nt guide strand exhibits strikingly preferential affinity for AGO10, the closest AGO1 paralog. The 22-nt miR168-AGO10 complex antagonizes AGO1 accumulation in part via "transitive RNAi", a silencing-amplification process, to maintain appropriate AGO1 cellular homeostasis. Furthermore, we found that the tombusviral P19 silencing-suppressor protein displays markedly weaker affinity for the 22-nt form among its isomiR168 cargoes, thereby promoting AGO10-directed suppression of AGO1-mediated antiviral silencing. Taken together, these findings indicate that structural flexibility, a previously overlooked property of pre-miRNAs, considerably increases the versatility and regulatory potential of individual MIRNA genes, and that some pathogens might have evolved the capacity or mechanisms to usurp this property.
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