ArticleMolecular microbiology2018
Formation of functional, non-amyloidogenic fibres by recombinant Bacillus subtilis TasA.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Molecular mechanisms and applications of antimicrobial secondary metabolites ofApplied and environmental microbiology · 2026Review
- Microplate-based quantification of poly-γ-glutamic acid levels in biofilm samples.Access microbiology · 2026Article
- The Importance of Being Imperfect: Structure and Function of Bacterial Amyloid.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Probiotic-Based Materials as Living Therapeutics.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- The Role of Biofilm-Derived Compounds in Microbial and Protozoan Interactions.Microorganisms · 2025Review
- Metabolic Engineering Strategy forFoods (Basel, Switzerland) · 2025Review
- Cloning, Heterologous Expression, and Antifungal Activity Evaluation of a Novel Truncated TasA Protein fromInternational journal of molecular sciences · 2025Article
- Article
- The role of filamentous matrix molecules in shaping the architecture and emergent properties of bacterial biofilms.The Biochemical journal · 2024Review
- Lateral interactions govern self-assembly of the bacterial biofilm matrix protein BslA.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Molecular characterization of the N-terminal half of TasA during amyloid-like assembly and its contribution to Bacillus subtilis biofilm formation.NPJ biofilms and microbiomes · 2023Article
- TapA acts as specific chaperone in TasA filament formation by strand complementation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Systematic microscopical analysis reveals obligate synergy between extracellular matrix components duringBiofilm · 2022Article
- Donor-strand exchange drives assembly of the TasA scaffold in Bacillus subtilis biofilms.Nature communications · 2022Article
- Group II truncated haemoglobin YjbI prevents reactive oxygen species-induced protein aggregation ineLife · 2022Article
- The ins and outs of Bacillus proteases: activities, functions and commercial significance.FEMS microbiology reviews · 2022Review
- Bacillus subtilis biofilm formation and social interactions.Nature reviews. Microbiology · 2021Review
- Fibrilar Polymorphism of the Bacterial Extracellular Matrix Protein TasA.Microorganisms · 2021Article
- Bacterial extracellular matrix as a natural source of biotechnologically multivalent materials.Computational and structural biotechnology journal · 2021Review
- Emerging Roles of Functional Bacterial Amyloids in Gene Regulation, Toxicity, and Immunomodulation.Microbiology and molecular biology reviews : MMBR · 2020Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Bacterial biofilms are communities of microbial cells encased within a self-produced polymeric matrix. In the Bacillus subtilis biofilm matrix, the extracellular fibres of TasA are essential. Here, a recombinant expression system allows interrogation of TasA, revealing that monomeric and fibre forms of TasA have identical secondary structure, suggesting that fibrous TasA is a linear assembly of globular units. Recombinant TasA fibres form spontaneously, and share the biological activity of TasA fibres extracted from B. subtilis, whereas a TasA variant restricted to a monomeric form is inactive and subjected to extracellular proteolysis. The biophysical properties of both native and recombinant TasA fibres indicate that they are not functional amyloid-like fibres. A gel formed by TasA fibres can recover after physical shear force, suggesting that the biofilm matrix is not static and that these properties may enable B. subtilis to remodel its local environment in response to external cues. Using recombinant fibres formed by TasA orthologues we uncover species variability in the ability of heterologous fibres to cross-complement the B. subtilis tasA deletion. These findings are indicative of specificity in the biophysical requirements of the TasA fibres across different species and/or reflect the precise molecular interactions needed for biofilm matrix assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.