ArticleScientific reports2018
Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
77 citing papers in PubMed, 147 citations in OpenAlex.
- Methods for measuring neural activity during voluntary wheel running.Journal of neuroscience methods · 2026Article
- Simultaneous two- and three-photon multiplane imaging across cortical layers in freely moving mice.Nature methods · 2026Article
- A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo.bioRxiv : the preprint server for biology · 2026Article
- Miniaturized widefield microscope for high speed in vivo voltage imaging.Biomedical optics express · 2026Article
- Long-Stokes-Shift mScarlet3 as a Structural Marker for Two-Photon Imaging.microPublication biology · 2026Article
- High-speed neural imaging with multiplexed miniaturized two-photon microscopy.Cell reports methods · 2025Article
- High-throughput two-photon volumetric brain imaging in freely moving mice.Nature communications · 2025Article
- A versatile miniature two-photon microscope enabling multicolor deep-brain imaging.Nature methods · 2025Article
- Nonmechanical spectral domain optical coherence tomography using an electrowetting beam-scanner.Optics express · 2025Article
- Miniaturized widefield microscope for high speed in vivo voltage imaging.bioRxiv : the preprint server for biology · 2025Article
- Open-source, high performance miniature 2-photon microscopy systems for freely behaving animals.Nature communications · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Focus-tunable two-photon fiberscope enablingOptics letters · 2025Article
- Alleviating head-mounted weight burden for neural imaging in freely-behaving rodents.Scientific reports · 2025Article
- The role of electroencephalography in epilepsy research-From seizures to interictal activity and comorbidities.Epilepsia · 2025Review
- Article
- Integrating Optics and Parametrically-Resonant Micro-Scanner Design for Large Working Distance Implantable Microscopy.IEEE/ASME transactions on mechatronics : a joint publication of the IEEE Industrial Electronics Society and the ASME Dynamic Systems and Control Division · 2025Article
- Review
- Wearable optical coherence tomography angiography probe with extended depth of field.Journal of biomedical optics · 2025Article
- Opto2P-FCM: A MEMS based miniature two-photon microscope with two-photon patterned optogenetic stimulation.bioRxiv : the preprint server for biology · 2024Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We present a miniature head mounted two-photon fiber-coupled microscope (2P-FCM) for neuronal imaging with active axial focusing enabled using a miniature electrowetting lens. We show three-dimensional two-photon imaging of neuronal structure and record neuronal activity from GCaMP6s fluorescence from multiple focal planes in a freely-moving mouse. Two-color simultaneous imaging of GFP and tdTomato fluorescence is also demonstrated. Additionally, dynamic control of the axial scanning of the electrowetting lens allows tilting of the focal plane enabling neurons in multiple depths to be imaged in a single plane. Two-photon imaging allows increased penetration depth in tissue yielding a working distance of 450 μm with an additional 180 μm of active axial focusing. The objective NA is 0.45 with a lateral resolution of 1.8 μm, an axial resolution of 10 μm, and a field-of-view of 240 μm diameter. The 2P-FCM has a weight of only ~2.5 g and is capable of repeatable and stable head-attachment. The 2P-FCM with dynamic axial scanning provides a new capability to record from functionally distinct neuronal layers, opening new opportunities in neuroscience research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.